Regulation of lung EC transcriptome by leene in mouse
Ontology highlight
ABSTRACT: Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of LEENE in vivo, particularly in the context of hypertension, we subjected WT and leene-KO mice to an Angiotensin II-induced hypertension model. We then profiled the transcriptomic changes in the EC-enriched fractions of the lung.
Project description:Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of LEENE in leene-KO mice and delineate the contribution of LEENE RNA in the ischemic response, we supplemented the leene-KO mice with human LEENE RNA and profiled the transcriptomic changes in the EC-enriched fractions of the ischemic muscles.
Project description:To test the function of LEENE in leene-KO mice and delineate the contribution of LEENE RNA in the ischemic response, we supplemented the leene-KO mice with human LEENE RNA and performed single cell RNA-seq. The transcriptome of the EC-enriched fractions in the ischemic muscles was profiled at the single cell level.
Project description:Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of human long non-coding RNA LEENE in Human umbilical vein endothelial cells (HUVEC), we inhibited LEENE by using a previously validated locked nucleic acid (LNA) GapmeR under pulsatile flow which mimics the physiological state and is characterized by elevated LEENE levels. PolyA-enriched RNA-seq was performed to examine the LEENE-regulated gene expression.
Project description:Chromatin-associated long non-coding RNAs (ca-lncRNAs) play important roles in transcriptional regulation. To comprehensively investigate the chromatin interactome of an enhancer-derived ca-lncRNA, LEENE, we performed the Chromatin isolation by RNA purification (ChIRP) to pull down LEENE-associated DNAs, which were subjected to DNA-seq. Specifically, we used 10 previously validated tiling nucleotides targeting different domains based on the predicted secondary structure. LEENE was overexpressed by adenovirus to enhance the ChIRP-seq signals.
Project description:To understand molecular changes underlying vascular pathology initiated by endothelial Sox17 deficiency, we performed endothelial-specific transcriptomic profiling using highly endothelial-enriched transcripts from the lung of control and Sox17i∆EC/hypoxic mice
Project description:We report the application of RNA sequencing in lung tissues of MCT rats, that were treated with Angiotensin 2 or TRV023 or Losartan to study their effects on Pulmonary hypertension. We received FPKM data of each gene from each group of MCT rats. The gene expression of the study MCT rats were normalized to control MCT rat. The differentially expressed genes in Angiotensin 2 and TRV023 treated MCT rats, confirmed that like Angiotensin 2, TRV023 is also involved in vascular remodelling and lead to worsening of pulmonary hypertension.