Transcriptomics

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Next Generation Sequencing Facilitates Quantitative Analysis of transcriptomes in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells sorted from the bone marrow of NSG mice transplanted with these cells


ABSTRACT: To determine what signalling pathways are affected by LILRB1 in MM cells, ARP-1 MM cell lines were transfected with lentivirus to knockdown LILRB1, injected to nsg mice, sorted from the bone marrow of NSG mice and sent for RNA-seq. Total RNAs of 2 x 10^6 CTR-KD ARP-1 cells or LILRB1-KD ARP-1 cells were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Cancer Genomics Center at The University of Texas (Houston, TX) for RNA-seq followed by data analysis. We use the RNA-seq data to determine differential expression of genes in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226821 | GEO | 2024/03/07

REPOSITORIES: GEO

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