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Small RNA-mediated genetic switches coordinate ALG-3/4 small RNA pathway function

ABSTRACT: Coordination of gene regulatory networks is necessary for proper execution of cellular programs throughout development. RNA interference (RNAi) is an essential regulatory mechanism in all metazoans. Proper RNAi-mediated gene regulation requires coordination of several RNAi branches to ensure homeostasis. For example, in C. elegans, the Argonautes, ALG-3 and ALG-4, are expressed specifically during spermatogenesis (L4 stage) and bind small interfering RNAs (siRNAs) complementary to sperm-enriched genes. We find that alg-3 and alg-4 are regulated by siRNAs. Our work shows that gene switches are operated via these siRNAs to regulate the Argonautes’ expression in a temporal manner. This RNAi-to-RNAi regulatory cascade is essential for coordinating ALG-3/4 pathway function, particularly during heat stress, to provide thermotolerant sperm-based fertility. This work provides insight into one regulatory motif used to maintain RNAi homeostasis, across developmental stages, despite environmental stressors. As RNAi pathways are evolutionarily conserved, other species likely use similar regulatory architectures to maintain RNAi homeostasis.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE226891 | GEO | 2024/06/25

REPOSITORIES: GEO

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ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline

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Analysis of transgene siRNAs and ARL-8-dependent siRNAs in Caenorhabditis elegans

2013-12-15 | E-GEOD-52785 | biostudies-arrayexpress

Caenorhabditis elegans

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| PRJNA152313 | ENA

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