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ScRNA-Seq analysis of HSC, MPP1 and MPP2 cells after serial transplantation of miR-221/222-proficient and deficient HSC.

ABSTRACT: We have studied the effect of miR-221/222-deficiency on early hematopoietic stem and progenitor compartments after serial transplantation of miRNA-proficient and deficient HSC.

ORGANISM(S): Mus musculus

PROVIDER: GSE227322 | GEO | 2023/08/21

REPOSITORIES: GEO

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scRNA-Seq analysis of HSC, MPP1 and MPP2 cells after serial transplantation of miR-221/222-proficient and deficient HSC.

Project description:scRNA-Seq analysis of HSC, MPP1 and MPP2 cells after serial transplantation of miR-221/222-proficient and deficient HSC.

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Antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast cancer cells

Project description:Full title: Expression data from antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast cancer cells The expression of miR-221/222 were found to be upregulated in fulvestrant resistant breast cancer cells MCF7-FR compared to its drug-sensitive counterpart MCF7. To investigate the role of miR-221/222 in acquired resistance to fulvestrant, we lowered the level of miR-221/222 in MCF7-FR cells using miRNA inhibitors (antagomirs), and compared gene expression profiles before and after treatment. Fulvestrant-resistant breast cancer cells MCF7-FR (originated from drug-sentitive breast cancer model cell line MCF7) were transient-transfected by antigomirs targeting miR221 or miR222 (i.e. si221, si222). All three cell lines, MCF7-FR, siR221, siRNA222 were subjected to gene expression profiling.

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miR-221/222 drive synovial fibroblast expansion and pathogenesis of TNF-mediated arthritis

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