Transcriptomics

Dataset Information

0

Reading and writing of mRNA m6A modification orchestrate mouse maternal-to-zygotic transition


ABSTRACT: Background: Maternal and zygotic mRNAs play critical roles in maternal-to-zygotic transition (MZT) stage and N6-methyladenosine (m6A) has been proven as an important RNA metabolism regulation system. However, the dynamic profiles of m6A modification during mammalian MZT and its potential functions remain largely unknown. Results: Here we utilized m6A-seq, low-input RNA-seq, LiRibo-seq and low-input proteomic analysis to examine the mRNA regulation dynamics during mouse MZT. We found that m6A could be inherited from maternal origin or de novo added after fertilization. Interestingly, we observed the de novo m6A modification on mRNA during zygotic genome activation (ZGA), especially at major ZGA. Further analysis showed that m6A modification on maternal mRNAs not only correlates with mRNA degradation after fertilization, but also maintains the stability of a small group of mRNAs thereby promoting their translation after fertilization. Using CRISPR-Cas13d mediated RNA editing, we systemically evaluated the functions of ten m6A regulators and identified Ythdc1 and Ythdf2 as key m6A readers for mouse preimplantation development. By combining low-input RNA-seq, RNA immunoprecipitation (RIP) and qRT-PCR, we uncovered and verified that Ythdc1 can associate with a small group of m6A-tagged mRNAs and participate in their mRNA stability regulation. Conclusions: Our integrated multi-omic analysis links m6A modification to the diverse fates of maternal and zygotic mRNAs and establishes a key role of m6A mediated RNA metabolism in mammalian MZT.

ORGANISM(S): Mus musculus

PROVIDER: GSE228201 | GEO | 2023/03/28

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-09-22 | E-GEOD-47558 | biostudies-arrayexpress
2023-03-27 | PXD041023 | Pride
2024-05-01 | PXD036678 | Pride
2017-03-27 | PXD006098 | Pride
2013-09-22 | GSE47558 | GEO
2023-05-23 | PXD041399 | Pride
2021-11-28 | GSE169632 | GEO
2023-02-10 | GSE192440 | GEO
2019-11-12 | GSE128283 | GEO
2021-09-09 | PXD018794 | Pride