Transcriptomics

Dataset Information

0

Hidden features of NAD-RNA epitranscriptome in Drosophila life cycle


ABSTRACT: Nicotinamide adenine dinucleotide (NAD), a nucleotide-containing metabolite, can be incorporated into the RNA 5’-terminus to result in NAD-capped RNA (NAD-RNA). Since NAD has been heightened as one of the most essential metabolites in cells, its attachment to RNA might indicate a yet-to-be discovered mechanism at the epitranscriptomic level. Here, we design a highly-sensitive method, DO-seq, to capture NAD-RNAs. Using Drosophila, we identify thousands of previously unexplored NAD-RNAs and their dynamics in the fly life cycle, from embryo to adult. We show the evidence that chromosomal clustering might be the structural basis by which co-expression can couple with NAD capping on physically and functionally-linked genes. Furthermore, we note that NAD capping of cuticle genes seems to inversely correlate with gene expression. Combined, we propose NAD-RNA epitranscriptome as a hidden layer of regulation that profoundly impacts biological processes. DO-seq empowers the identification of NAD-capped RNAs, facilitating functional investigation into this modification.

ORGANISM(S): Mus musculus Drosophila melanogaster

PROVIDER: GSE228505 | GEO | 2023/12/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-01-27 | GSE194271 | GEO
2023-12-20 | GSE226636 | GEO
2019-06-03 | GSE127002 | GEO
2020-11-23 | GSE142389 | GEO
2020-11-23 | GSE142388 | GEO
| PRJNA800040 | ENA
2019-05-20 | GSE110801 | GEO
2021-04-21 | GSE153253 | GEO
2023-03-17 | GSE217259 | GEO
2017-03-09 | GSE90884 | GEO