Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Knockdown of LTB4R, HCAR3, GPR153, and GPR137 in primary human keratinocytes

ABSTRACT: RNA sequencing (RNAseq) of primary neonatal human keratinocytes transduced with pooled siRNAs targeting LTB4R, HCAR3, GPR153, and GPR137, or non-targeting control siRNA (siCon)

ORGANISM(S): Homo sapiens

PROVIDER: GSE228651 | GEO | 2023/07/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

KLF4 knockdown in human keratinocytes

Project description:RNA sequencing (RNAseq) of N/TERT2G keratinocytes transduced with pooled siRNAs targeting KLF4, or non-targeting control siRNA (siCon)

2020-02-24 | GSE137410 | GEO

YAP1 and TAZ knockdown in human keratinocytes

Project description:RNA sequencing (RNAseq) of N/TERT2G keratinocytes transduced with pooled siRNAs targeting YAP1 and TAZ (WWTR1), or non-targeting control siRNA (siCon)

2020-02-24 | GSE137531 | GEO

TEAD transcriptional inhibition in basal cell carcinoma

Project description:RNA sequencing (RNAseq) of primary keratinocytes from mouse basal cell carcinoma mice (BCC) transduced with pooled siRNAs targeting YAP1 and TAZ (siYT) or non-targeting control siRNA (siCon), and with TEADi or GFP as control

2021-06-09 | GSE156913 | GEO

Down-regulation of MYC and TP63 in human keratinocytes

Project description:This transcriptome study is intended to discover the effects of MYC and TP63 on human keratinocytes differentiation at the genome level. Human keratinocyte cell line, HaCaT cells were transfected by siRNAs with the help of transfection reagent, INTERFERin. 48 hours post-transfection, total RNA was extracted from cells. We used a non-targeting siRNA as a negative control. siRNA targeting MYC or TP63 was obtained from QIAGEN, and RNAs from wild type HaCaT cells were used as reference samples. We did 3 biological replicates and 2 'dye swap' for each sample. Totally we got 12 samples, with 2 dye-swap for each sample, and 48 arrays used for all hybridization.

2010-05-15 | E-GEOD-17394 | biostudies-arrayexpress

A global transcriptome analysis of human epidermal keratinocytes upon knockdown of hsa_circ_0084443

Project description:Hsa_circ_0084443 expression level is down-regulated during normal skin wound healing and higher level of hsa_circ_0084443 was found in chronic non-healing diabetic foot ulcers compared to normal wounds. However, the biological function of hsa_circ_0084443 in epidermal keratinocytes during wound repair has not been studied. To study the genes regulated by hsa_circ_0084443, we transfected siRNA targeting hsa_circ_0084443 diagnostic junction into human primary epidermal keratinocytes to knockdown hsa_circ_0084443 expression. We performed a global transcriptome analysis of keratinocytes upon knockdown of hsa_circ_0084443 using Affymetrix arrays.

2019-10-01 | GSE114236 | GEO

Knockdown TIMM13 in A549 lung cancer cell line

Project description:We depleted TIMM13 using short interfering RNA (siRNA) in A549 lung cancer cells, and found that silencing of TIMM13 could significantly inhibit A549 cell proliferation. Thus, we conducted RNA-seq in the A549 cells transduced with either nontargeting siRNA or two distinct TIMM13-specific siRNAs. We observed widespread gene expression change in A549 cells upon TIMM13 knockdown.

2023-06-17 | GSE234780 | GEO

Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes

Project description:We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects. Primary human keratinocytes were electroporated with scrambled control siRNA, a pair of siRNA oligomers against Erk1 and Erk2 (set A), or an independent pair of siRNA oligomers against Erk1 and Erk2 (set B). Four days after electroporation, RNA was harvested. Three biological replicates were performed for each of the three siRNA groups.

2009-05-03 | E-GEOD-15417 | biostudies-arrayexpress

Gene expression in human umbilical cord endothelial cells following FoxP1 knockdown

Project description:To search for genes regulated by the transcription factor Forkhead box Protein P1 (FoxP1) in endothelial cells, we transfected human umbilical cord endothelial cells (HUVECs) with a combination of three siRNA oligonucleotides directed against human FoxP1 . Human umbilical vein endothelial cells (HUVECs) were isolated from donated umbilical cords, pooled from two donors and cultivated up to passage 5. For transfection with FoxP1-siRNA cells were cultured to 70% confluence and transfected with a combination of three FoxP1-targeting siRNAs or an irrelevant control oligonucleotide (all from invitrogen) using Lipofectamin RNAiMax (Invitrogen) according to the manufacturers instructions. Total RNA was isolated 48h after transfection.

2013-11-12 | GSE32968 | GEO

Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes

2009-05-03 | GSE15417 | GEO

Identification of lncRNA-NTT regulated genes using small interference RNA knockdown for cDNA microarray

Project description:We used microarrays to detail the global programme of gene expression underlying lncRNA-NTT knockdown We employed small interference RNA (siRNA) and microarray techniques to investigate the effect of NTT silencing on gene expression in HepG2 cells. HepG2 cells were transfected with NTT siRNA (siNTT) or control siRNA (siCon) for 120 h, and then RNA extraction and cDNA synthesis for hybridization on Affymetrix microarrays. We collect siRNA-transfection HepG2 cells and test knockdown efficiency. After comparison of the siCon group then we choose the best three sample for microarray.

2014-08-01 | E-GEOD-59957 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data