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Single-cell RNA sequencing analysis of metaphocytes and epidermal cells

ABSTRACT: Metaphocytes are tissue-resident myeloid-like cells of non-hematopoietic origin in zebrafish barrier tissues. How metaphocytes acquire myeloid cell properties from non-hematopoietic precursors during the development remains unknown. Here we show that metaphocytes are in situ generated from local progenitors guided by the ETS transcription factor Spic. Tg(spic:EGFP) transgenic fish were found to label both metaphocytes and their progenitor cells. To reveal the genetic programs underlying the development of metaphocytes, we performed single-cell RNA sequencing analysis of spic:GFP+ cells in the epidermis of adult Tg(spic:EGFP) fish. The result further supports that mature metaphocytes are generated from spic+ progenitors via an intermediate immature state and gradually obtain their myeloid characteristics during this process.

ORGANISM(S): Danio rerio

PROVIDER: GSE228737 | GEO | 2023/04/08

REPOSITORIES: GEO

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Single-cell RNA sequencing analysis of metaphocytes and epidermal cells

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