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Novel time-resolved reporter mouse reveals spatial and transcriptional heterogeneity during alpha cell differentiation

ABSTRACT: Glucagon-expressing pancreatic α cells have attracted much attention for their plasticity to transdifferentiate into insulin-producing β cells; however, it remains unclear precisely when and where α cells emerge from and what regulates α-cell fate. We therefore explored the spatial and transcriptional heterogeneity of 　α-cell differentiation by a novel time-resolved reporter system. We established the mouse model, ‘Gcg-Timer’, in which newly generated α cells can be distinguished from more differentiated cells by their fluorescence. Transcriptome analysis of Gcg-Timer embryos revealed that the mRNAs related to angiogenesis were enriched in newly generated-α cells. Novel time-resolved analysis with Gcg-Timer reporter mouse uncovered spatiotemporal features of α-cell neogenesis, which will enhance our understanding of cellular identity and plasticity within the islets.

ORGANISM(S): Mus musculus

PROVIDER: GSE229090 | GEO | 2023/09/15

REPOSITORIES: GEO

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