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Transplanting FVIII/ET3-Secreting Cells in Fetal Sheep Increases FVIII Levels Long-Term Without Inducing Immunity or Toxicity

ABSTRACT: To investigate the differentiative fate of human PLCs following transplantation into fetal sheep and engraftment in various tissues/organs, we performed gene expression profiling analysis using data obtained from RNA-seq of human PLCs prior to in utero transplantation and of each engrafted fetal sheep tissue after filtering to remove any cross-reactivity with orthologous sheep transcripts

ORGANISM(S): Homo sapiens Ovis aries

PROVIDER: GSE230081 | GEO | 2023/06/01

REPOSITORIES: GEO

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Effects of Maternal Energy Intake on Genomic Imprinting in Fetal Sheep

Project description:Background: Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parental origin. To date a total of 255 imprinted genes have been identified or predicted among all investigated mammal species. However, only 21 have been described in sheep and 11 are annotated in current ovine genome. Results: Here we aim to use DNA/RNA throughput sequencing to identify monoallelically expressed and imprinted genes in organs of day 135 fetal sheep, and 2) to determine whether different levels of maternal energy intake (100% of NRC energy requirement or control, 140% or over-, and 60% or under-fed) influence genomic imprinting. We report strategies to solve technical challenges in the next-generation sequencing data analysis pipeline, including alignment bias of RNA sequencing reads and filtering potential false positives. We identified 80 monoallelically expressed and 18 putatively imprinted genes using the list of 255 stated above as a guide. Five (45.6% of 11) were already known imprinted genes in sheep, the other 13 were known imprinted in other mammals. Sanger sequencing confirmed four putative sheep imprinted genes INPP5F, PLAGL1, CASD1 and PPP1R9A. Among the 13 putative imprinted genes, five localized in the known sheep imprinting clusters of MEST domain on chromosome 4, DLK1/GTL2 domain on chromosome 18 and IGF2/H19 domain on chromosome 21, three were in a novel sheep imprinted cluster on chromosome 4 known in other species as PEG10/SGCE. Additionally, we found that the imprinted genes PHLDA2, SLC22A18, DIRAS3, and IGF2 were differentially expressed, albeit without allelic expression reversal, among the three maternal nutritional groups. Conclusions: Together, our results expanded the sheep imprinted gene list to 34 and demonstrated the influence of maternal diet on fetal imprinting under the conditions studied.

2018-08-29 | GSE111306 | GEO

Expression data from fetal sheep immunocytes

Project description:study investigating the initiation of systemic inflammatory signaling in fetuses exposed to TLR-4 agonist lipopolysaccharides from E.coli microarray was used to determine the inflammatory transcriptome in circulating fetal immunocytes fetal sheep at 92d GA were catheterised prior to intraamniotic exposure fo saline or lipopolysaccharides for 48h prior to euthanasia

2015-08-21 | E-GEOD-72240 | biostudies-arrayexpress

High expression of adrenal cortisol synthases is acquired after intrauterine inflammation in periviable sheep fetuses

Project description:To clarify the effects of endotoxin-induced intrauterine inflammation on the HPA axis of periviable sheep fetuses, fetal sheep (0.63 term) were divided into two groups: (1) the endotoxin group, in which the endotoxin was injected into the amniotic fluid and (2) the control group, in which the saline solution was injected instead. Gene expression levels in the fetal adrenal glands of each group were analyzed by RNA-seq.

2023-07-28 | GSE230267 | GEO

Expression data from uterus tissue of sheep

Project description:Embryo implantation is an essential step for the establishment of pregnancy and is crucial for the successful embryo transplantation of in vitro fertilization embryos. The successful implantation of an embryo depends upon cellular and molecular dialog between the uterus and the embryo. We used microarrays to detail the key regulated genes underlying embryo implantation process in sheep.

2016-07-15 | GSE84417 | GEO

RNA Sequencing of Pancreatic Islets from Intrauterine Growth Restricted and Normally-Grown Near Term Sheep Fetuses

Project description:The study objective was to determine differentially expressed mRNA transcripts in pancreatic islets from fetal sheep with placental insufficiency-induced intrauterine growth restricted.

2017-09-08 | GSE90022 | GEO

Lipidomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-II)

Project description:Cardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis

2018-09-10 | ST001061 | MetabolomicsWorkbench

Transplanting FVIII/ET3-Secreting Cells in Fetal Sheep Increases FVIII Levels Long-Term Without Inducing Immunity or Toxicity

Project description:Transplanting FVIII/ET3-Secreting Cells in Fetal Sheep Increases FVIII Levels Long-Term Without Inducing Immunity or Toxicity

| PRJNA957430 | ENA

NMR Metabolomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-I)

Project description:Cardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis

2018-08-31 | ST001060 | MetabolomicsWorkbench

RNA Sequencing of Perirenal Adipose Tissue from Intrauterine Growth Restricted and Normally-Grown Near Term Sheep Fetuses with and without intact adrenal medulla.

Project description:The study objective was to determine differentially expressed mRNA transcripts in perirenal adipose tissue from fetal sheep that underwent sham or adrenal demedullation prior to fetal growth restriction to determine the role of catecholamines on gene expression following placental insufficiency-induced intrauterine growth restriction.

2023-12-05 | GSE132734 | GEO

RNAseq of Islets Isolated from Near Term Fetal Sheep after 7-day Infusion of Norepinephrine or Vehicle Control

Project description:The study objective was to determine differentially expressed mRNA transcripts in pancreatic islets from fetal sheep exposed to persistent elevations in stress hormone norepinephrine to isolate effects of chronic adrenergic signaling on fetal islets. Ultimately, these data can be compared to islets from fetuses with placental insufficiency-induced intrauterine growth restriction, where there is known increases in norepinephrine.

2018-09-05 | GSE106440 | GEO

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