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Project description:Liver Cullin 3 knockout transcriptomics
| PRJNA961690 | ENA
Project description:To get further insights into the processes leading to attenuation of early stage atherosclerosis in the bortezomib treated LDLR-KO mice, microarray profiling of gene expression was performed on RNA taken from whole aortae of bortezomib treated LDLR-KO and sham- treated LDLR-KO mice fed a Western diet for 6 weeks and compared the changes in gene expression in both groups to non-atherosclerotic CD animals. Male 10-week-old LDLR-KO mice (B6.129S7-Ldlrtm1Her/J; JAX Mice, Boston) were fed a Western-type diet for 6 weeks ad libitum and received intraperitoneal injections of bortezomib (50 µg/kg body weight; WD+Bor) or saline (WD) twice weekly (n = 4). Mice that were fed normal diet served as chow diet control (CD; n = 4). Gene expression in aortic tissue was measured. Two independent experiments were performed.
2011-07-13 | E-GEOD-30638 | biostudies-arrayexpress
Project description:Non-alcoholic fatty liver disease is strongly associated with obese and type 2 diabetes. It has been reported that an oxidized cholesterol, 7-ketocholesterol (7KC) might cause inflammatory response in macrophages and plasma 7KC concentration were higher in patients with cardiovascular diseases or diabetes. Therefore, we have decided to test whether small amount of 7KC in diet might induce hepatic steatosis and inflammation in two types of obese models. We found that addition of 0.01% 7KC either in chow diet (CD, regular chow diet with 1% cholesterol) or western type diet (WD, high fat diet with 1% cholesterol) accelerated hepatic neutral lipid accumulation by Oil Red O staining. Importantly, by lipid extraction analysis, it has been recognized that triglyceride rather than cholesterol species was significantly accumulated in CD+7KC compared to CD as well as in WD+7KC compared to WD. Immunostaining revealed that macrophages infiltration was increased in CD+7KC compared to CD and also in WD+7KC compared to WD. These phenotypes were accompanied by inducing inflammatory response and downregulating fatty acid oxidation. Furthermore, RNA sequence analysis demonstrated that 7KC reduced expression of genes which related to autophagy process. Levels of LC3-II protein were decreased in WD+7KC compared to WD. Similarly, we have confirmed the effect of 7KC on acceleration of steatohepatitis in db/db mice model. Collectively, our study has demonstrated that small amount of dietary 7KC contributed to accelerate hepatic steatosis and inflammation in obese mice models.
2021-01-15 | GSE159234 | GEO
Project description:A heterodimer of ATP-binding cassette transporters G5 and G8 (ABCG5/G8) functions as a lipid exporter in the intestine. Homozygous ABCG5/G8 knockout female mice exhibit infertility under normal chow diet conditions due to impaired folliculogenesis and poor egg quality. Interestingly, this infertility was restored by changing the diet from a chow diet (CD) to a vegetable oil-free diet (VOFD). To elucidate mechanisms underlying the diet-induced infertiility in ABCG5/G8 KO mice, RNA-seq analyses were conducted and found that multiple genes potentially involved in the reproductive system and embryogenesis, in addition to those involved in sterol biosynthesis, were down regulated in the ovaryes from CD-fed infertile KO mice compared to those from fertile CD-fed wild-type mice and VOFD-fed KO mice.
2024-10-25 | GSE279573 | GEO
Project description:To identify molecular mechanism underlying the protection from diet-induced hepatic steatosis in AHNAK deficiency mice, we examined microarray analysis with liver sample from HFD-fed AHNAK KO and WT mice. Two-condition experiment, regular chow (CD) -fed WT vs. CD-fed AHNAK KO and High fat diet(HFD)-fed WT vs. HFD-fed AHNAK KO mice. Biological replicates: 3 control, One replicate per array.
2015-06-23 | E-GEOD-70119 | biostudies-arrayexpress
Project description:Male and female mice (Bl6/J) were fed a chow diet (control 1 and control 2) or a High fat diet (HFD) or a Choline deficient High fat diet (CD HFD) or a Western Diet (WD) or a Western Diet supplemented with glucose and fructose in drinking water (WD glucose fructose) for 15 weeks.
2022-10-31 | GSE159090 | GEO
Project description:We performed RNA sequencing of the aortic valves of C57BL/6J (the wild type with chow diet), Ldlr-/- (1. with chow diet, 2. with WD), and Apoe-/- mice (1. with chow diet, 2. with WD) to compare the overall transcriptomic characteristics within genetic differences (wild type, Ldlr-/-, Apoe-/-) or in dietary differences (chow diet versus WD).
2022-08-22 | GSE205587 | GEO
Project description:To further understanding the function of cullin 3 during inflammation in macrophages, we have employed mouse bone marrow derived macrophages microarray expression profiling to identify the gens that involve in regulationg inflammatory respones upon LPS challenge. Mouse BMM were stimulated with LPS for 6 hours and RNA was extracted for microarray. Ogt was indentified by comparison between wildtype and Cullin 3 knockout BMM.
2017-01-06 | GSE93166 | GEO
Project description:Consumption of a diet rich in saturated fatty acids and carbohydrates contributes to the accumulation of fat in the liver and development of non-alcoholic steatohepatitis (NASH). Herein we investigated the hypothesis that short-term consumption of a high fat/sucrose Western diet (WD) alters the genomic and translatomic profile of the liver in association with changes in signaling through the protein kinase mTORC1, and that such alterations contribute to development of NAFLD. The results identify a plethora of mRNAs that exhibit altered expression and/or translation in the liver of rats consuming a WD compared to a CD. In particular, consumption of a WD altered the abundance and ribosome association of mRNAs involved in lipid and fatty acid metabolism, as well as those involved in glucose metabolism and insulin signaling. Hepatic mTORC1 signaling was enhanced when rats were fasted overnight and then refed in the morning; however, this effect was blunted in rats fed a WD as compared to a CD. Despite similar plasma insulin concentrations, fatty acid content was elevated in the liver of rats fed a WD as compared to a CD. We found that feeding had a significant positive effect on ribosome occupancy of 49 mRNAs associated with hepatic steatosis (e.g., LIPE, LPL), but this effect was blunted in the liver of rats fed a WD. In many cases, changes in ribosome association were independent of alterations in mRNA abundance, suggesting a critical role for diet-induced changes in mRNA translation in the expression of proteins encoded by those mRNAs. Overall, the findings demonstrate that short-term consumption of a WD impacts hepatic gene expression by altering the abundance of many mRNAs, but also causes wide-spread variation in mRNA translation that potentially contribute to development of hepatic steatosis.
2022-10-17 | GSE214513 | GEO
Project description:We report the single cell transcriptome of CD4+ T cells from atherosclerotic aortas from ApoE-deficient mice that have been fed a western diet (WD) with aggravated atherosclerosis or with a standard chow diet (CD) with moderate atherosclerosis. We show that aortic T cells have a unique transcriptome with a mixed phenotype overlapping with T-regulatory and pathogenic T-helper type -1 and -17 cells.
2020-05-21 | GSE149069 | GEO