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Single-cell transcriptome and TCR repertoire sequencing of cholinergic CD4+ T cells in liver cancer

ABSTRACT: ACh was originally isolated from spleen back in 1929, however, its contribution to immune regulation has only recently been appreciated. Subsets of both T and B lymphocytes have been found to express choline acetyltransferase (Chat) and produce ACh. To date, Chat-expressing T cells have been described as relaying neural signals in the spleen to modulate immune responses; regulating blood pressure; and promoting anti-viral immune responses. In a murine multi-hit model of hepatocellular carcinoma, we observed activation of the adaptive immune response and induction of Chat-expressing CD4+ T cells. These cholinergic T cells curtail the development of liver cancer by supporting anti-tumor immune responses. We used single-cell RNA sequencing to investigate the transcriptome and TCR repertoire of the Chat-expressing CD4+ T cells in healthy and HCC-bearing livers.

ORGANISM(S): Mus musculus

PROVIDER: GSE231322 | GEO | 2023/06/26

REPOSITORIES: GEO

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Cholinergic regulation of thymocyte negative selection

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