Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Refining the transcriptional maps of Pseudomonas aeruginosa phages using ONT-cappable-seq

ABSTRACT: The global transcriptional profiles of Pseudomonas aeruginosa phages LUZ19, LUZ24, YuA, PAK_P3, 14-1 and phiKZ was obtained using the long read RNA sequencing technique ONT-cappable-seq. Using this approach we obtained a comprehensive genome-wide map of viral transcription start sites, terminators and transcription units.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE231702 | GEO | 2024/03/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages

Project description:We developed ONT-cappable-seq, a specialized long-read RNA sequencing technique that allows end-to-end sequencing of primary prokaryotic transcripts using the Nanopore sequencing platform. We applied ONT-cappable-seq to study the transcriptional landscape of Pseudomonas aeruginosa phage LUZ7, leading to a comprehensive genome-wide map of viral transcription start sites, terminators and complex operon structures that fine-regulate gene expression. At the same time, it provides new insights in the RNA biology of LUZ7 and paves the way for more in depth transcription studies that can help unveil the complex layers of phage-host interactions.

2022-06-24 | GSE196845 | GEO

Transcriptomics-driven characterization of novel T7-like temperate Pseudomonas phage LUZ100

Project description:The global transcriptional profile of novel T7-like Pseudomonas aeruginosa phage LUZ100 was obtained using the long read RNA sequencing technique ONT-cappable-seq. Using this approach we obtained a comprehensive genome-wide map of viral transcription start sites, terminators and transcription units and gained new insights in the molecular mechanisms of transcriptional regulation of T7-like temperate phages.

2023-02-08 | GSE211961 | GEO

ONT-cappable-seq for P23-45 bacteriophage 5 min post-infection

Project description:We performed the long-read RNA sequencing technique ONT-cappable-seq on RNA samples of T. thermophilus infected with phage P23-45 5 minutes post-infection. Using this approach, we obtained the primary transcriptome at the early infection stage and sequenced it in full-length. Based on this data, we were able to identify viral transcription start sites and termination sites and uncover distinct promoter motifs.

2023-12-12 | GSE215811 | GEO

Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages

Project description:Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages

| PRJNA807530 | ENA

Assessing the transcriptional landscape of Pseudomonas chlororaphis phage 201f2-1: uncovering the small regulatory details of a giant phage

Project description:The global transcriptional profile of Pseudomonas chlororaphis infecting phage 201f2-1 was obtained using the long-read RNA sequencing technique ONT-cappable-seq. this resulted in a comprehensive genome-wide map of viral transcription start and termination sites. In addition, we were able to identify different transcription units and gained new insights in the molecular mechanisms of of transcriptional regulation of members of the Phikzvirus.

2024-10-17 | GSE245572 | GEO

Phages DT1 and 2972 gene expression study

Project description:This series represents the gene expression study of phages DT1 and 2972 during the whole process of infection. Gene expression was measured at nine time intervals (0, 2, 7, 12, 17, 22, 27, 32, 37 minutes) during phage infection.

2005-08-10 | E-GEOD-2476 | biostudies-arrayexpress

Salmonella phages

Project description:Salmonella phages

| PRJEB31250 | ENA

unclassified phages

Project description:unclassified phages

| PRJNA16918 | ENA

Butyrivibrio phages

Project description:Butyrivibrio Phages

| PRJNA613207 | ENA

Enrichment of Wolbachia Endosymbiont Transcripts from Host Nematode RNA by Cappable-seq

Project description:We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.

2017-01-05 | GSE93139 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data