Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Massively-parallel Microbial mRNA Sequencing (M3-Seq) reveals heterogenous behaviors in bacteria at single-cell resolution

ABSTRACT: M3-Seq is a single-cell RNA-sequencing platform for bacteria that pairs combinatorial cell indexing with post hoc rRNA depletion. We use M3-Seq to profile hundreds of thousands of bacterial cells and reveal rare populations of bacteria that include bet-hedging strategies, prophage induction, and phage-infected cells in E. coli and B. subtilis.

ORGANISM(S): Escherichia coli Pseudomonas aeruginosa Bacillus subtilis

PROVIDER: GSE231935 | GEO | 2023/07/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Massively-parallel Microbial mRNA Sequencing (M3-Seq) reveals heterogenous behaviors in bacteria at single-cell resolution

Project description:Massively-parallel Microbial mRNA Sequencing (M3-Seq) reveals heterogenous behaviors in bacteria at single-cell resolution

| PRJNA970263 | ENA

Molecular mechanism of muscarinic acetylcholine receptor M3 interaction with Gq

Project description:Muscarinic acetylcholine receptor M3 (M3) and its downstream effector Gq/11 are critical drug development targets given their involvement in numerous physiological processes and diseases. Although a cryo-electron microscopy study previously defined the structure of the M3-miniGq complex, the lack of information on the intracellular loop 3 (ICL3) of M3 and α-helical domain (AHD) of Gαq has made it difficult to comprehend the molecular mechanism of M3-Gq coupling fully. Here, we present the molecular mechanism underlying the dynamic interactions between the wild-type full-length M3 and heterotrimeric Gq using hydrogen-deuterium exchange mass spectrometry and NanoLuc Binary Technology-based cell systems. This study suggests potential binding interfaces between M3 and Gq in pre-assembled and fully active nucleotide-free complexes. In addition to well-known binding interfaces, we observed the interaction of long ICL3 with Gβγ. Furthermore, M3 ICL3 negatively affected M3-Gq coupling, and the Gαq AHD underwent unique conformational changes during M3-Gq coupling. Therefore, we propose a comprehensive molecular mechanism of M3-Gq coupling by analyzing previously well-defined binding interfaces and neglected regions, such as M3 ICL3 and the Gαq AHD.

2024-05-22 | PXD042562 | Pride

Bacteria strain:M3-1

Project description:Bacteria strain:M3-1 Genome sequencing

| PRJNA1130115 | ENA

Vibrio anguillarum M3 strain:M3

Project description:Vibrio anguillarum M3 strain:M3 Genome sequencing

| PRJNA211964 | ENA

Mycobacterium tuberculosis M3 strain:M3

Project description:Mycobacterium tuberculosis M3 strain:M3 Genome sequencing

| PRJNA183185 | ENA

Streptomyces sp. M3 strain:M3

Project description:Streptomyces sp. M3 strain:M3 Genome sequencing and assembly

| PRJNA448824 | ENA

Microbacterium sp. M3 strain:M3

Project description:Microbacterium sp. M3 strain:M3 Genome sequencing and assembly

| PRJNA1034264 | ENA

Mantle cell lymphoma gene expression profiling array VFN-M3.

Project description:Mantle cell lymphoma gene expression profiling array VFN-M3. The dataset contains data from 10 samples hybridized on Illumina HumanHT-12 array. -patient's primary lymphoma cells obtained from leukemized blood (P6-PBMC1, P6-PBMC2) -non-malignant B-cells isolated from peripheral blood of 5 healthy donors (VFN-M3-CTRL1, VFN-M3-CTRL2) -lymphoma cell line named UPF7U established from the patient's primary lymphoma cells after 166 and 226 days of in vitro cultivation (UPF7U-D166, UPF7U-D226) -cells established by xenotransplantation of UPF7U cell line isolated ex vivo from subcutaneously growing lymphoma (UPF7U-SC) -patient-derived xenograft (PDX) cells named VFN-M3 established by xenotransplantation of primary lymphoma cells into immunodeficient mice isolated ex vivo from the lymph node like tumor (VFN-M3-LN3) -patient-derived xenograft (PDX) cells named VFN-M3 established by xenotransplantation of primary lymphoma cells into immunodeficient mice isolated ex vivo from subcutaneously growing lymphoma (VFN-M3-SC1, VFN-M3-SC3). Samples were sorted using CD19-microbeads (Miltenyi). For xenotransplantation, NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (referred to as NSG mice) were used.

2017-02-24 | GSE95291 | GEO

Vibrio anguillarum M3 strain:M3

Project description:Listonella anguillarum M3 genome sequencing

| PRJNA51953 | ENA

A comparison of transcriptome between Drosophila S2R+ cell line cultured in M3 media supplemented with fly extract and S2R+ cell line cultured in M3 + BPYE + 10% FCS

Project description:We report the transcriptome analysis of Drosophila S2R+ cells cultured in media lacking vertebrate fetal bovine serum during log-phase of growth. The three media conditions are: (1) M3 BPYE 10% FCS (2) M3 + 10% Fex and (3) M3 + 2.5% Fex.

2020-12-22 | GSE154485 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data