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Rapid retinoic acid-induced trophectoderm model from human induced pluripotent stem cells

ABSTRACT: A limited number of accessible and representative models of human trophoblast cells currently exist for disease modeling and improving our understanding of placentation. Current stem cell models involve either a transition through a naïve stem cell fate or precise dynamic control of multiple interacting growth factor and small molecule cues. Here, we show that simple exposure of hiPSCs to two small molecules, retinoic acid (RA) and the Wnt signaling agonist CHIR 99021 (CHIR), results in rapid, synergistic upregulation of CDX2, an important transcription factor for trophectoderm cell induction and maintenance of the trophoblast cell population. RA+CHIR-treated cells have a transcriptional profile with high similarity to that of primary trophectoderm cells, and they can also be further differentiated into cells with features of syncytiotrophoblasts and extravillous trophoblasts. We assessed RA+CHIR-treated cells for the established set of criteria defining a trophectoderm cell model, and the cells possess all of the features necessary to be considered a valid model. Taken together, our data demonstrate the development of a simple method for the generation of functional trophoblast-like cells that can be used to better understand mechanisms and interactions involving the placenta.

ORGANISM(S): Homo sapiens

PROVIDER: GSE232692 | GEO | 2024/08/15

REPOSITORIES: GEO

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Project description:The trophoblast cell lineage is specified as early as the blastocyst stage, leading to the individualization of trophectoderm from pluripotent cells of the inner cell mass. We used a double in vitro transcription mRNA amplification technique and compared trophectoderm with pluripotent stem cells. These five trophectoderm samples were compared to 176 human embryonic stem cell samples and adult samples that are publicly available in GEO under the following accession numbers: GSM136511 GSM136512 GSM136513 GSM136519 GSM136525 GSM136526 GSM172579 GSM175789 GSM175792 GSM175795 GSM175810 GSM175811 GSM175812 GSM175814 GSM175815 GSM175817 GSM175818 GSM175820 GSM175821 GSM175825 GSM175826 GSM175827 GSM175842 GSM175843 GSM175849 GSM175850 GSM175852 GSM175853 GSM175859 GSM175860 GSM175861 GSM175862 GSM175863 GSM175864 GSM175866 GSM175867 GSM175869 GSM175870 GSM175871 GSM175872 GSM175874 GSM175875 GSM175876 GSM175879 GSM175880 GSM175885 GSM175886 GSM175901 GSM175902 GSM175906 GSM175907 GSM175908 GSM175911 GSM175935 GSM175937 GSM175941 GSM175942 GSM175943 GSM175948 GSM175949 GSM175950 GSM175951 GSM175967 GSM175974 GSM175975 GSM175977 GSM175978 GSM175979 GSM175982 GSM175984 GSM175985 GSM175987 GSM175988 GSM175993 GSM175999 GSM176000 GSM176002 GSM176003 GSM176004 GSM176006 GSM176007 GSM176009 GSM176010 GSM176012 GSM176017 GSM176026 GSM176028 GSM176058 GSM176113 GSM176115 GSM176119 GSM176123 GSM176131 GSM176136 GSM176243 GSM176245 GSM176265 GSM176268 GSM176269 GSM176276 GSM176281 GSM176282 GSM176283 GSM176284 GSM176285 GSM176286 GSM176300 GSM176301 GSM176310 GSM176318 GSM176320 GSM176321 GSM176322 GSM176323 GSM176325 GSM176326 GSM176328 GSM176328 GSM176329 GSM176330 GSM176331 GSM176332 GSM176333 GSM176334 GSM176336 GSM176337 GSM176338 GSM176344 GSM176345 GSM176346 GSM176352 GSM176353 GSM176363 GSM176365 GSM176381 GSM176382 GSM176385 GSM176386 GSM176387 GSM176389 GSM176390 GSM176391 GSM176407 GSM176408 GSM176411 GSM176412 GSM176413 GSM176430 GSM176431 GSM176432 GSM176433 GSM176435 GSM183605 GSM183606 GSM183607 GSM183608 GSM183609 GSM194307 GSM194308 GSM245341 GSM248200 GSM248201 GSM248202 GSM248204 GSM249025 GSM249027 GSM257524 GSM288812 GSM288876 GSM288877 GSM388581 GSM388636 GSM388638 GSM452261 GSM452691 GSM453652

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Rapid retinoic acid-induced trophectoderm model from human induced pluripotent stem cells

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