Transcriptomics

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The transcriptome profiling of multi-cell types by the droplet based full-length single-cell transcript sequencing


ABSTRACT: We developed a scalable Tn5-based Full-Length In situ Transcriptome sequencing method (scFLIT-seq). This method combines the advantages of in situ reverse transcription of methanol-fixed cells, single-cell separation and barcode ligation via the 10X scATAC-seq protocol and microfluidic platform, and Tn5-mediated fragmentation and tagging of cDNA. Various cells treated with different concentrations of methanol were used to validate the efficacy of this method. The efficacy at the single-cell level of this method was validated on a mixed cell population of HEK293T and NIH/3T3 cells.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE233170 | GEO | 2024/01/01

REPOSITORIES: GEO

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