Transcriptomics

Dataset Information

0

The transcriptome profiling of multi-cell types by the droplet based full-length single-cell transcript sequencing


ABSTRACT: We developed a scalable Tn5-based Full-Length In situ Transcriptome sequencing method (scFLIT-seq). This method combines the advantages of in situ reverse transcription of methanol-fixed cells, single-cell separation and barcode ligation via the 10X scATAC-seq protocol and microfluidic platform, and Tn5-mediated fragmentation and tagging of cDNA. Various cells treated with different concentrations of methanol were used to validate the efficacy of this method. The efficacy at the single-cell level of this method was validated on a mixed cell population of HEK293T and NIH/3T3 cells.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE233170 | GEO | 2024/01/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-05-16 | E-MTAB-11467 | biostudies-arrayexpress
2019-06-04 | GSE126465 | GEO
| PRJNA1153325 | ENA
2023-05-15 | GSE209947 | GEO
2022-10-06 | GSE214723 | GEO
2021-04-06 | GSE165181 | GEO
2019-02-11 | GSE105452 | GEO
2024-01-09 | E-MTAB-13181 | biostudies-arrayexpress
2015-01-01 | GSE63424 | GEO
2024-04-14 | GSE215901 | GEO