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RNA sequencing analysis in Caco2 cells infected with vector or PIM1

ABSTRACT: PIM1 is constitutively active and involved in various cellular processes, including cell survival, proliferation, and differentiation by phosphorylating a wide range of substrates. We investigated whether PIM1 is involved in the pathogenesis of ulcerative colitis (UC).We performed RNA sequencing analysis in Caco2 cells infected with vector or PIM1 to study the molecular mechanisms through which PIM1 regulates goblet cell numbers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE234122 | GEO | 2024/07/30

REPOSITORIES: GEO

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ChIP-seq analysis for HDAC2 in Caco2 cells infected with vector or PIM1

Project description:PIM1 is constitutively active and involved in various cellular processes, including cell survival, proliferation, and differentiation by phosphorylating a wide range of substrates. We investigated whether PIM1 is involved in the pathogenesis of ulcerative colitis (UC).We performed ChIP-seq analysis for HDAC2 to study the molecular mechanisms through which PIM1 regulates goblet cell numbers.

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RNA sequencing analysis in Caco2 cells infected with vector or PIM1

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Analysis of MYC- and PIM1-dependent genes

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Project description:We show that METTL3 expression is decreased in the colon mucosa in ulcerative colitis (UC) patients, and its expression is strongly associated with the differentiation and maturation of goblet cells during inflammation.Depletion of METTL3 significantly inhibits the self-renewal and differentiation of Lgr5+ stem cells, especially the differentiation and maturation of goblet cells, results in intestinal dysplasia and spontaneous inflammation.

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