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Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (HuGene-1_0-st)

ABSTRACT: Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors

ORGANISM(S): Homo sapiens

PROVIDER: GSE23413 | GEO | 2011/08/04

SECONDARY ACCESSION(S): PRJNA133341

REPOSITORIES: GEO

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Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (HuGene-1_0-st)

Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors Five samples analyzed.

2011-08-03 | E-GEOD-23413 | biostudies-arrayexpress

Reprogramming of embryonic human fibroblasts into fetal hematopoietic progenitors by fusion with human fetal liver CD34+ cells.

Project description:Experiments with somatic cell nuclear transfer, inter-cellular hybrid formation_ENREF_3, and ectopic expression of transcription factors have clearly demonstrated that cell fate can be dramatically altered by changing the epigenetic state of cell nuclei. Here we demonstrate, using chemical fusion, direct reprogramming of the genome of human embryonic fibroblasts (HEF) into the state of human fetal liver hFL CD34+ (hFL) hematopoietic progenitors capable of proliferating and differentiating into multiple hematopoietic lineages. We show that hybrid cells retain their ploidy and can differentiate into several hematopoietic lineages. Hybrid cells follow transcription program of differentiating hFL cells as shown by genome-wide transcription profiling. Using whole-genome single nucleotide polymorphism (SNP) profiling of both donor genomes we demonstrate reprogramming of HEF genome into the state of hFL hematopoietic progenitors. Our results prove that it is possible to convert the fetal somatic cell genome into the state of fetal hematopoietic progenitors by fusion. This suggests a possibility of direct reprogramming of human somatic cells into tissue specific progenitors/stem cells without going all the way back to the embryonic state. Direct reprogramming of terminally differentiated cells into the tissue specific progenitors will likely prove useful for the development of novel cell therapies.

| S-EPMC3077375 | biostudies-literature

Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (GenomeWideSNP_6)

Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors

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Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (RNA-Seq)

Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors

2011-08-04 | GSE23415 | GEO

Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (GenomeWideSNP_6)

Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors Two samples analyzed.

2011-08-03 | E-GEOD-23414 | biostudies-arrayexpress

Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (RNA-Seq)

Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors One sample analyzed.

2011-08-04 | E-GEOD-23415 | biostudies-arrayexpress

Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-08-04 | GSE23416 | GEO

Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-08-04 | E-GEOD-23416 | biostudies-arrayexpress

γH2A.X Modulates Self-renewal and Differentiation of Human Pluripotent Stem Cells. [HuGene-1_0-st]

Project description:Histone variants are important epigenetic regulators known to play a role in governing the processes of self-renewal and lineage specific differentiation . Phosphorylation of the histone variant H2A.X (γH2A.X) has historically been associated with DNA damage response, but more recent investigations have further demonstrated it’s role in cell cycle, aging and early development. Through both genetic and chemical targeting approaches, we now reveal a direct involvement of γH2A.X in hPSC self-renewal and differentiation decisions in vitro and in vivo. Namely, reduction of γH2A.X levels enhance hPSC differentiation toward mesodermal derivatives with concomitant inhibition of ectodermal derivatives. In contrast, ectopic expression of a constitutively phosphorylated mimic of γH2A.X enhanced hPSCs differentiation toward ectodermal fate while mesodermal differentiation was hindered. These phenotypic observations were associated with γH2A.X occupancy in the promoter regions of key regulatory genes associated with pluripotency and lineage choice. Our study suggests γH2A.X is a functional epigenetic pluripotency marker and novel target for guiding cell-fate commitment during differentiation.

2021-12-06 | GSE92643 | GEO

Cancer Associated Fibroblasts are defined by a core set of epigenome changes that contribute to the tumor phenotype [HuGene-1_0-st]

Project description:Cancer Associated Fibroblasts (CAFs) play an active role in tumourigenesis. It is unknown how the permanent phenotypic changes in CAFs are encoded at the molecular level. Here we use whole genome sequencing and microarray analysis to interrogate the epigenome, transcriptome and genome of patient-matched CAF and non-malignant prostate fibroblast (NPF) cells.

2018-03-26 | GSE86256 | GEO

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