ERK5 sustains autocrine mitogenic balance for cell fate specification in human pluripotent stem cells [XMD RNA-seq]
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ABSTRACT: To investigate the function ERK5 in the regulation of hPSC pluripotency, we processed ERK5 inhibition (XMD892) treamtent in E8 medium for 12/24/48hr in H1 ESCs.
Project description:To investigate the function ERK5 in the regulation of hPSC histone modification, we processed ERK5 inhibition (XMD892) treamtent in E8 medium for 24hr in H1 ESCs. And H3K27ac and H3K4me3 ChIP-seq test
Project description:To investigate the function ERK5 in the regulation of hPSC pluripotency, we processed ERK5 inhibition (XMD892) treamtent in E8 medium for 12hr in H1 ESCs.
Project description:To investigate the function of MEK5/ERK5 in the regulation of hPSC pluripotency, we processed MAP2K5DD (MEK5DD) overexpression H1 ESC line.
Project description:To investigate the function ERK5 in the regulation of mitogen expression in multiple cell lines, we processed RNA-seq analysis of H1/H9 ESCs and NL1/NL4 hiPSCs lines.
Project description:To investigate the function glycolysis in the regulation of hPSC pluripotency, we processed glycolysis inhibition (2DG) treamtent in E8 medium for 5 cell passages in H1/H9 ESCs and NL1/NL4 hiPSCs.