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Reproductive aging: fem-1 day 3 oocyte vs fem-1 day 8 oocyte

ABSTRACT: To find genes in C. elegans oocytes associated with reproductive aging.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE23447 | GEO | 2010/09/01

SECONDARY ACCESSION(S): PRJNA133337

REPOSITORIES: GEO

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Reproductive aging: sma-2;fem-1 day 8 oocyte vs fem-1 day 8 oocyte

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Reproductive aging: fem-1 day 3 oocyte vs fem-1 day 8 oocyte

Project description:To find genes in C. elegans oocytes associated with reproductive aging. Five replicates comparing RNA from oocyte samples collected from day 3 fem-1(hc17) animals with RNA from oocyte samples collected from day 8 fem-1(hc17) animals. Three out of five are dye-flipped.

2010-09-01 | E-GEOD-23447 | biostudies-arrayexpress

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Project description:Chromosome segregation errors in oocytes lead to the production of aneuploid eggs, which are the leading cause of pregnancy loss and of several congenital diseases such as Down syndrome. The frequency of chromosome segregation errors in oocytes increases with maternal age, especially at a late stage of reproductive life. How aging at various life stages affects oocytes differently remains poorly understood. In this study, we describe aging-associated changes in the transcriptome profile of mouse oocytes throughout reproductive life. Our single-oocyte comprehensive RNA sequencing using RamDA-seq revealed that oocytes undergo transcriptome changes at a late reproductive stage, whereas their surrounding cumulus cells exhibit transcriptome changes at an earlier stage. Calorie restriction, a paradigm that reportedly prevents aging-associated egg aneuploidy, promotes a transcriptome shift in oocytes with the up-regulation of genes involved in chromosome segregation. This shift is accompanied by the improved maintenance of chromosomal cohesin, the loss of which is a hallmark of oocyte aging and causes chromosome segregation errors. These findings have implications for understanding how oocytes undergo aging-associated functional decline throughout their reproductive life in a context-dependent manner.

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Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [GV]

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2010-09-01 | E-GEOD-23509 | biostudies-arrayexpress

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