Transcriptomics

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CBFB-DSP-ChIPseq and YAP-DSP-ChIPseq


ABSTRACT: We developed a new method to extract open-chromatins that contain trans-acting factors (TAFs)-DNA complex crosslinked with dithiobis(succinimidyl propionate) (DSP). Using this method we identified CBFB as a cell density-sensitive TAF, which translocates between the cytoplasm and the nucleus in cell density-dependent manner. Here, we demonstrate that the extracted TAF/DNA complex is also amenable to ChIP-seq analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE234731 | GEO | 2023/12/05

REPOSITORIES: GEO

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