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Early-responsive molecular signatures associated with halophytic adaptation in Sesuvium portulacastrum (L.)

ABSTRACT: Sesuvium portulacastrum (L.) is a halophyte, adapted to grow naturally under high-saline environments. Under control conditions, an inverse correlation was seen between Na and K accumulation, suggesting its facultative nature and ability to use Na-K interchangeably. No significant growth reduction was seen in seedlings upto 250 mM NaCl, except for youngest leaf-curling. Significantly higher accumulation of proline (4.3/1.8-folds), glycine betaine (1.4/1.4-folds) and selected amino-acids (1.4-4.2/1.4-2.3-folds) were seen in root/shoot of seedlings within 8 h stress duration. Inspite of having higher Na-accumulation, significantly lower number of differentially expressed genes (DEGs) were identified at 24 h than 8 h stress duration, indicating transcriptional restoration. As initial hours after NaCl stress are dominated by osmotic-component, 8h-specific DEGs, which are mainly transporters and transcription factors, reflect plant’s response towards NaCl-induced osmotic challenge. Most of the growth-related pathways including photosynthesis and ribosome-associated biogenesis gets suppressed, to support the activation of stress defence. Overexpression of SpRAB18 (an ABA-responsive dehydrin), one of the top-ranked DEGs, was found to impart salt-tolerance in soybean, indicating gene-function level conservation between halophyte and glycophyte. An open-access transcriptome database “SesuviumKB” (https:/ /cb.imsc.res.in/sesuviumkb) was developed to enable wide-scale gene function studies in S. portulacastrum, that could pave the way to engineer salt-tolerance in crops.

ORGANISM(S): Sesuvium portulacastrum

PROVIDER: GSE235196 | GEO | 2024/06/16

REPOSITORIES: GEO

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Project description:Bacteria respond to osmotic stress by a substantial increase in the intracellular osmolality, adjusting their cell turgor for altered growth conditions. Using E. coli as a model organism we demonstrate here that bacterial responses to hyperosmotic stress specifically depend on the nature of osmoticum used. We show that increasing acute hyperosmotic NaCl stress above ~1.0 Os kg-1 causes a dose-dependent K+ leak from the cell, resulting in a substantial decrease in cytosolic K+ content and a concurrent accumulation of Na+ in the cell. At the same time, isotonic sucrose or mannitol treatment (non-ionic osmotica) results in a gradual increase of the net K+ uptake. Ion flux data is consistent with growth experiments showing that bacterial growth is impaired by NaCl at the concentration resulting in a switch from net K+ uptake to efflux. Microarray experiments reveal that about 40% of up-regulated genes shared no similarity in their responses to NaCl and sucrose treatment, further suggesting specificity of osmotic adjustment in E. coli to ionic- and non-ionic osmotica The observed differences are explained by the specificity of the stress-induced changes in the membrane potential of bacterial cells highlighting the importance of voltage-gated K+ transporters for bacterial adaptation to hyperosmotic stress. Experiment Overall Design: Two biological replicates per treatment with microarray analysis using the Affymetrix GeneChip E. coli Genome array. Treatments used included: Experiment Overall Design: Control - E. coli Frag1, grown to early stationary growth phase in a mineral salts medium with 0.1% glucose at 25 C Experiment Overall Design: Sucrose hyperosmotic treatment - 1.25 M sucrose added to control culture Experiment Overall Design: for 10 min. Experiment Overall Design: NaCl hyperosmotic treatment - 1.37 M NaCl added to control culture Experiment Overall Design: For microarray data comparisons the sucrose and NaCl hyperosmotic treatment data was compared to the no treatment control data separately. The sucrose to control and NaCL to control comparison data tables are linked below.

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Early-responsive molecular signatures associated with halophytic adaptation in Sesuvium portulacastrum (L.)

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