Transcriptomics

Dataset Information

0

Structure of an aberrant spliceosome intermediate on its way to disassembly


ABSTRACT: Intron removal during pre-mRNA splicing is of extraordinary complexity and its disruption causes a vast number of genetic diseases in humans. While key steps of the canonical spliceosome cycle have been revealed by combined structure-function analyses, structural information on an aberrant spliceosome committed to discard is not available. Here, we report the cryo-EM structure of a B state spliceosome intermediate primed for disassembly. We identify the DEAH-box helicase – G patch protein pair (Gih35-Gpl1) to maintain catalytic dormancy with Gpl1 recognizing a remodeled active site of the spliceosome due to a single-nucleotide insertion at the 3’ end of the 5’ exon. Remodeling is communicated to the spliceosome surface and the Ntr1 complex is recruited. Our data pave the way for a targeted analysis of spliceosome-associated quality control.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE235589 | GEO | 2024/06/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA986513 | ENA
2019-11-12 | PXD011542 | Pride
2018-02-19 | GSE108059 | GEO
2019-08-30 | E-MTAB-6950 | biostudies-arrayexpress
2019-08-03 | E-MTAB-8182 | biostudies-arrayexpress
| PRJEB27592 | ENA
2024-02-04 | GSE246283 | GEO
2015-09-03 | E-GEOD-66182 | biostudies-arrayexpress
2014-02-19 | E-GEOD-53647 | biostudies-arrayexpress
| PRJNA739885 | ENA