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The gene profile analysis of the function of HMGA2 in immortalized human ovarian surface epithelial cell lines.

ABSTRACT: To identify HMGA2-regulated downstream target genes, we compared the difference of gene profiles between ovarian surface epithelial cells without HMGA2 (T29-1, T29-2, T80) and cells with HMGA2 (T29A2-8, T29A2-15, T80A2-11).

ORGANISM(S): Homo sapiens

PROVIDER: GSE23599 | GEO | 2010/08/14

SECONDARY ACCESSION(S): PRJNA131743

REPOSITORIES: GEO

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The gene profile analysis of the function of HMGA2 in immortalized human ovarian surface epithelial cell lines.

Project description:To identify HMGA2-regulated downstream target genes, we compared the difference of gene profiles between ovarian surface epithelial cells without HMGA2 (T29-1, T29-2, T80) and cells with HMGA2 (T29A2-8, T29A2-15, T80A2-11). Immortalized ovarian surface epithelial cells (named T29-1, T29-2, T80) were transfected with HMGA2 retrovirus vector pBabe-HMGA2 (named T29A2-8, T29A2-15, T80A2-11). Then cells were harvested for RNA isolation for gene expression analysis. Control group: T29-1, T29-2, T80. Testing group: T29A2-8, T29A2-15, T80A2-11.

2010-08-14 | E-GEOD-23599 | biostudies-arrayexpress

The microRNA profile analysis of the function of HMGA2 in immortalized human ovarian surface epithelial cell lines

Project description:To identify HMGA2-regulated downstream target microRNAs, we compared the difference of microRNA profiles between ovarian surface epithelial cells without HMGA2 (T29) and cells with HMGA2 (T29A2). Immortalized ovarian surface epithelial cells (named T29) were transfected with HMGA2 retrovirus vector pBabe-HMGA2 (named T29A2). Then cells were harvested for RNA isolation for microRNA expression analysis.

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Ovarian Cancer gene expression profiling identifies the surface of the ovary as a stem cell niche

Project description:In contrast to epithelial derived carcinomas that arise in most human organs, ovarian surface epithelial cells become more rather than less differentiated as the malignancy progresses. To test the hypothesis that ovarian surface epithelial cells retain properties of relatively uncommitted pluripotent cells until undergoing neoplastic transformation, we conducted gene expression profiling analysis (Affymetrix, U133 Plus 2.0) of 12 ovarian surface epithelial cells and 12 laser capture microdissected serous papillary ovarian cances. We find that over 2000 genes are significantly differentially expressed between the surface epithelial and cancer samples. Network analysis implicates key signaling pathways and pathway interactions in ovarian cancer development. Genes previously associated with adult stem cell maintenance are expressed in ovarian surface epithelial cells and significantly down-regulated in ovarian cancer cells. Our results indicate that the surface of the ovary is an adult stem cell niche and that deregulation of genes involved in maintaining the quiescence of ovarian surface epithelial cells is instrumental in the initiation and development of ovarian cancer.

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