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Sciatic nerve injury-responsive differential mRNA profile and ribosome association efficiency of injury-induced mRNA

ABSTRACT: After nerve injury in the pheripheral nervous system, axon regeneration occurs through transcriptomic changes in neuronal cell bodies. To investigate which protein-coding transcripts are up-regulated and directed to ribosomes or not, we used RiboTag+/+;Advillin-Cre+ mice, expressing HA-tagged RPL22 specifically in sensory neurons, to profile their ribosome binding RNAs in nerve injured or uninjured dorsal root ganglion(DRG) tissues using HA-IP sequencing. We analyzed the results together with total RNA transcriptomics conducted under the same conditions. Through this analysis, we found mRNA of Gpr151 gene, one of the transcript that highly responds to injury but does not direct to the ribosomes, promoting axon regeneration by a non-coding RNA function.

ORGANISM(S): Mus musculus

PROVIDER: GSE237046 | GEO | 2023/08/18

REPOSITORIES: GEO

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Promoting axon regeneration by enhancing the non-coding function of the injury-responsive coding gene Gpr151

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Sciatic nerve injury-responsive differential CSDE1 association efficiency of ribosomal protein coding mRNAs

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