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Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation

ABSTRACT: Epigenetic regulation of chromatin states is crucial for proper gene expression programs and progression during development, but precise mechanisms by which epigenetic factors influence differentiation remain poorly understood. Here we find that the histone variant H2A.Z accumulates at Sox motif-containing promoters during zebrafish gastrulation while neighboring genes become transcriptionally active. These changes coincide with reduced expression of anp32e, the H2A.Z histone removal chaperone, suggesting that loss of Anp32e may lead to increases in H2A.Z binding during differentiation. Remarkably, genetic removal of Anp32e in embryos leads to H2A.Z accumulation prior to gastrulation and developmental genes become precociously active. Accordingly, H2A.Z accumulation occurs most extensively at Sox motif-associated genes, including many which are normally activated following gastrulation. Altogether, our results provide compelling evidence for a mechanism in which Anp32e preferentially restricts H2A.Z accumulation at Sox motifs to regulate the initial phases of developmental differentiation in zebrafish.

ORGANISM(S): Danio rerio

PROVIDER: GSE237444 | GEO | 2023/12/24

REPOSITORIES: GEO

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Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation

Project description:Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation

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