Transcriptional profiling of cortex from a mouse model of Huntington's disease (Q140) crossed with Msh3 heterozygous and homozygous mice [Msh3_cortex_6m]
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ABSTRACT: Cortex was isolated from 6-month-old Msh3+/- and Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Msh3+/-, Q140 X Msh3+/-, Msh3-/-, and Q140 X Msh3-/-, 7-9 replicates per genotype.
Project description:Striatum was isolated from 12-month-old Msh3+/- and Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (snRNASeq) was performed on 6 genotypes: WT, Q140, Msh3+/-, Q140 X Msh3+/-, Msh3-/-, and Q140 X Msh3-/-, 4 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Msh3+/- and Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Msh3+/-, Q140 X Msh3+/-, Msh3-/-, and Q140 X Msh3-/-, 7-8 replicates per genotype.
Project description:As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington’s disease (HD) through various computational modeling efforts. Striatum was isolated from 12-month-old Msh3+/- mice and Msh3-/- (KO) crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, 8 replicates per genotype.
Project description:As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington’s disease (HD) through various computational modeling efforts. Cerebral cortex was isolated from 12-month-old Msh3+/- mice and Msh3-/- (KO) crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, 8 replicates per genotype.
Project description:Striatum was isolated from 12-month-old Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Genomic analysis (ATACseq) was performed on 4 genotypes: WT, Msh3-/-, Het (Q140) KI, and Het (Q140) KI X Msh3-/-, 6 replicates per genotype.
Project description:Cortex was isolated from 6-month-old Pms2+/- and Pms2-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Pms2+/-, Q140 X Pms2+/-, Pms2-/-, and Q140 X Pms2-/-, 8 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Ccdc82+/- and Ccdc82-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Ccdc82+/-, Q140 X Ccdc82+/-, Ccdc82-/-, and Q140 X Ccdc82-/-, 7-8 replicates per genotype.
Project description:Cortex was isolated from 6-month-old Ccdc82+/- and Ccdc82-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Ccdc82+/-, Q140 X Ccdc82+/-, Ccdc82-/-, and Q140 X Ccdc82-/-, 7-8 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Pms2+/- and Pms2-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Pms2+/-, Q140 X Pms2+/-, Pms2-/-, and Q140 X Pms2-/-, 6-12 replicates per genotype.
Project description:Striatum was isolated from 4-month-old Mlh1+/- and Mlh1-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Mlh1+/-, Q140 X Mlh1+/-, Mlh1-/-, and Q140I X Mlh1-/-, 7-9 replicates per genotype.