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Response of osteoblasts on amine-based polymer coatings cor-relates with the amino group density

ABSTRACT: The integration of implants can be improved by physical but mainly by chemical modifications of the implant surface. It has already been shown that amine-based coatings improve cell attachment, cell migration, and intracellular signaling. The aim of this study was to determine the role of the amino group density in this positive cell response by developing controlled amino-rich nano-layers. Using Clariom S microarrays to perform genome-wide expression profilng, changes in adhesion related genes after cultivation of osteoblast-like cells on an amino group-rich coating could be seen.

ORGANISM(S): Homo sapiens

PROVIDER: GSE237945 | GEO | 2023/10/04

REPOSITORIES: GEO

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Project description:Nanowires (NWs), high-aspect-ratio nanomaterials, are increasingly used in technological materials and consumer products and may have toxicological characteristics distinct from nanoparticles. We carried out a comprehensive evaluation of the physicochemical stability of four silver nanowires (AgNWs) of two sizes and coatings and their toxicity to Daphnia magna. Inorganic aluminum-doped silica coatings were less effective than organic poly(vinyl pyrrolidone) coatings at preventing silver oxidation or Ag+ release and underwent a significant morphological transformation within 1 h following addition to low ionic strength Daphnia growth media. All AgNWs were highly toxic to D. magna but less toxic than ionic silver. Toxicity varied as a function of AgNW dimension, coating, and solution chemistry. Ag+ release in the media could not account for observed AgNW toxicity. Single-particle inductively coupled plasma mass spectrometry distinguished and quantified dissolved and nanoparticulate silver in microliter-scale volumes of Daphnia magna hemolymph with a limit of detection of approximately 10 ppb. The silver levels within the hemolymph of Daphnia exposed to both Ag+ and AgNW met or exceeded the initial concentration in the growth medium, indicating effective accumulation during filter feeding. Silver-rich particles were the predominant form of silver in hemolymph following exposure to both AgNWs and Ag+. Scanning electron microscopy imaging of dried hemolymph found both AgNWs and silver precipitates that were not present in the AgNW stock or the growth medium. Both organic and inorganic coatings on the AgNW were transformed during ingestion or absorption. Pathway, gene ontology, and clustering analyses of gene expression response indicated effects of AgNWs distinct from ionic silver on Daphnia magna. Four replicates each of five toxicant exposure groups of ~20 animals and four replicates of control, unexposed animals. Each control was compared to each exposed data set for a total of 16 comparisons per chemical condition.

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Response of osteoblasts on amine-based polymer coatings cor-relates with the amino group density

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