Lactobacillus plantarum IMDO 130201, a wheat sourdough isolate, adapts to growth in wheat sourdough simulation medium at different pH values through differential gene expression

ABSTRACT: Sourdough is a very competitive and challenging environment for microorganisms. Usually, a stable microbiota composed of lactic acid bacteria (LAB) and yeasts comes to dominate this ecosystem. Although rich in carbohydrates, thus providing an ideal environment to grow, the low pH presents a particular challenge. The nature of the adaptation to this low pH was investigated for Lactobacillus plantarum IMDO 130201, an isolate from a laboratory wheat sourdough fermentation. Batch fermentations were carried out in wheat sourdough simulation medium, and total RNA was isolated from mid-exponential growth phase cultures, followed by differential gene expression analysis using a LAB functional gene microarray. At low pH values, an increased expression of genes involved in peptide and amino acid metabolism was observed as well as of genes involved in plantaricin production and lipoteichoic acid synthesis. The results highlight cellular mechanisms that allow L. plantarum to function at a low environmental pH.

ORGANISM(S): Lacticaseibacillus casei Companilactobacillus farciminis Limosilactobacillus panis Weissella confusa Lactobacillus helveticus Latilactobacillus curvatus Lactococcus lactis Enterococcus mundtii Lentilactobacillus hilgardii Streptococcus thermophilus Lactobacillus gasseri Brevibacterium linens Pediococcus pentosaceus Limosilactobacillus pontis Lactobacillus crispatus Fructilactobacillus sanfranciscensis Lactobacillus acidophilus Latilactobacillus sakei Companilactobacillus alimentarius Lactiplantibacillus pentosus Lactobacillus delbrueckii Enterococcus faecium Bifidobacterium Lacticaseibacillus paracasei Oenococcus oeni Lentilactobacillus parabuchneri Fructilactobacillus fructivorans Companilactobacillus mindensis Leuconostoc mesenteroides Lentilactobacillus buchneri Companilactobacillus paralimentarius Lactiplantibacillus plantarum Lacticaseibacillus rhamnosus Furfurilactobacillus rossiae Limosilactobacillus fermentum Leuconostoc pseudomesenteroides Enterococcus faecalis Mammaliicoccus lentus Enterococcus casseliflavus Leuconostoc citreum Lactiplantibacillus paraplantarum Lactobacillus johnsonii Pediococcus acidilactici Lactobacillus amylovorus Staphylococcus aureus Enterococcus hirae Limosilactobacillus reuteri Levilactobacillus brevis Limosilactobacillus frumenti

PROVIDER: GSE23945 | GEO | 2011/08/01

SECONDARY ACCESSION(S): PRJNA130407

REPOSITORIES: GEO