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A somatic cell cloned rhesus monkey obtained by trophoblast replacement

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Macaca fascicularis Macaca mulatta

PROVIDER: GSE239746 | GEO | 2023/08/01

REPOSITORIES: GEO

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A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS I]

Project description:Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys (Macaca fascicularis), but the cloning of other non-human primate species remains to be achieved. On the other hand, our histological examination indicated severe calcification of the placenta of SCNT fetuses. Additionally, we found that some of the maternal-biased imprinting genes were aberrantly lost in the cloned embryos by multi-omics analysis. We thus developed a trophoblast replacement (TR) method, providing an ICSI-derived placenta for the SCNT fetus, to support the full-term development of the cloned embryos after implantation. By combining this TR method with treatment with epigenetic modification factors, we obtained a healthy somatic cell cloned rhesus monkey. Thus, TR represents a useful approach for rhesus monkey cloning.

2023-08-01 | GSE222930 | GEO

A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS II]

2023-08-01 | GSE239742 | GEO

A somatic cell cloned rhesus monkey obtained by trophoblast replacement [RNA-seq]

2023-08-01 | GSE239741 | GEO

A somatic cell cloned rhesus monkey obtained by trophoblast replacement

Project description:A somatic cell cloned rhesus monkey obtained by trophoblast replacement

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A somatic cell cloned rhesus monkey obtained by trophoblast replacement [RNA-seq]

Project description:A somatic cell cloned rhesus monkey obtained by trophoblast replacement [RNA-seq]

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A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS II]

Project description:A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS II]

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A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS I]

Project description:A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS I]

| PRJNA924042 | ENA

Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer.

Project description:Somatic cell nuclear transfer (SCNT) successfully clones cynomolgus monkeys, but the efficiency remains low due to a limited understanding of the reprogramming mechanism. Notably, no rhesus monkey has been cloned through SCNT so far. Our study conducts a comparative analysis of multi-omics datasets, comparing embryos resulting from intracytoplasmic sperm injection (ICSI) with those from SCNT. Our findings reveal a widespread decrease in DNA methylation and the loss of imprinting in maternally imprinted genes within SCNT monkey blastocysts. This loss of imprinting persists in SCNT embryos cultured in-vitro until E17 and in full-term SCNT placentas. Additionally, histological examination of SCNT placentas shows noticeable hyperplasia and calcification. To address these defects, we develop a trophoblast replacement method, ultimately leading to the successful cloning of a healthy male rhesus monkey. These discoveries provide valuable insights into the reprogramming mechanism of monkey SCNT and introduce a promising strategy for primate cloning.

| S-EPMC10791636 | biostudies-literature

Transcriptome analysis of rhesus monkey MII oocytes and embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-09-09 | GSE112537 | GEO

Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells.

Project description:BACKGROUND: Interspecies somatic cell nuclear transfer (iSCNT) has been proposed as a tool to address basic developmental questions and to improve the feasibility of cell therapy. However, the low efficiency of iSCNT embryonic development is a crucial problem when compared to in vitro fertilization (IVF) and intraspecies SCNT. Thus, we examined the effect of donor cell species on the early development of SCNT embryos after reconstruction with bovine ooplasm. RESULTS: No apparent difference in cleavage rate was found among IVF, monkey-bovine (MB)-iSCNT, and bovine-bovine (BB)-SCNT embryos. However, MB-iSCNT embryos failed to develop beyond the 8- or 16-cell stages and lacked expression of the genes involved in embryonic genome activation (EGA) at the 8-cell stage. From ultrastructural observations made during the peri-EGA period using transmission electron microscopy (TEM), we found that the nucleoli of MB-iSCNT embryos were morphologically abnormal or arrested at the primary stage of nucleologenesis. Consistent with the TEM analysis, nucleolar component proteins, such as upstream binding transcription factor, fibrillarin, nucleolin, and nucleophosmin, showed decreased expression and were structurally disorganized in MB-iSCNT embryos compared to IVF and BB-SCNT embryos, as revealed by real-time PCR and immunofluorescence confocal laser scanning microscopy, respectively. CONCLUSION: The down-regulation of housekeeping and imprinting genes, abnormal nucleolar morphology, and aberrant patterns of nucleolar proteins during EGA resulted in developmental failure in MB-iSCNT embryos. These results provide insight into the unresolved problems of early embryonic development in iSCNT embryos.

| S-EPMC2734572 | biostudies-literature

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