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ChIP-seq of human embryonic stem cells (hESCs) overexpressed with Monkeypox viral proteins H3L and A29L

ABSTRACT: We found that Monkeypox viral proteins H3L and A29L caused injuries in human cells. Whether they can bind chromatin and re-model chromation is unclear. Here, we applied ChIP-seq on hESCs to evaluate whether and how they are involved in chromatin regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE239888 | GEO | 2024/08/28

REPOSITORIES: GEO

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RNA-seq of cardiac lineage cells (day 3 of cardiac differentiation) derived from human embryonic stem cells (hESCs) overexpressed with Monkeypox virus-protein revealed the effects of H3L and A29L in human cardiac development.

Project description:Monkeypox virus (MPV) can infect human cells and induces injuries in human. However, whether and how MPV affect the function of human cells remain to be elucidated. Here, we overexpressed H3L and A29L in hESCs, separately. We appliced RNA-seq on cardiac lineage cells (day 3 of cardiac differentiation) derived from human embryonic stem cells (hESCs) overexpressed with Monkeypox virus-proteins (Control, H3L and A29L) and want to know gene expression patterns of control and overexpression cell lines. Subsequent data analyses will help us to know whether and how H3L and A29L affect human cardiac development.

2024-08-28 | GSE239712 | GEO

RNA-seq of Monkeypox virus-protein overexpression in human embryonic stem cells (hESCs) revealed the effects of H3L, A29L, A35R and I1L in human

Project description:Monkeypox virus (MPV) can infect human cells and induces injuries in human. However, whether and how MPV affect the function of human cells remain to be elucidated. Here, we overexpressed H3L, A29L, A35R and I1L in hESCs, separately. We appliced RNA-seq on these hESCs and want to know gene expression patterns of control and Overexpression hESCs. Subsequent data analyses will help us to know whether and how H3L, A29L, A35R or I1L affect cells in human.

2024-08-28 | GSE235128 | GEO

RNA-seq of mosue heart tissues overexpressed with Monkeypox virus (MPV) proteins H3L or A29L.

Project description:Here, we overexpressed Monkeypox virus (MPV) proteins H3L and A29L in mouse hearts, separately. We appliced bulk RNA-seq on heart muscle tissues (2 months after overexpression) and want to know gene expression patterns in control group and overexpression groups. Subsequent data analyses will help us to know whether and how H3L and A29L affect mosue heart.

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Infection of MK2 cells with monkeypox virus

Project description:Orthopox viruses, including monkeypox, multiply intracellularly and induce numerous changes in host genes expression. The virus target mainly humoral host response, and simultaneously, exploits other genes and functions to reproduce effectively. The goal of this experiment is to identify those host genes and functions that are essential for monkeypox virus replication. Mock infected control cells were treated and incubated identically to time point arms, except for virus exposure. Two time points of cells infected with monkeypox virus were harvested at 3, 7 hours post infection, and gene expression was assessed using microarray in all arms. The experiment was done in triplicate.

2010-04-07 | E-GEOD-21001 | biostudies-arrayexpress

Infection of MK2 cells with monkeypox virus

2010-03-25 | GSE21001 | GEO

Comparison non-targeting hRNA hESCs versus UBE2K shRNA hESCs

Project description:Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. When compared with their differentiated counterparts, immortal human embryonic stem cells (hESCs) have a unique chromatin architecture and low levels of trimethylated histone H3 at lysine 9 (H3K9me3), a heterochromatin-associated modification. Here we assess a link between the intrinsic epigenetic landscape and ubiquitin-proteasome system of hESCs. We find that hESCs exhibit high expression of UBE2K, a ubiquitin-conjugating enzyme. Loss of UBE2K increases the levels of H3K9 trimethyltransferase SETDB1, resulting in H3K9 trimethylation and repression of neurogenic genes during differentiation. Concomitantly, loss of UBE2K impairs the ability of hESCs to differentiate into neural progenitors with neurogenic properties. Besides H3K9 trimethylation, we find that UBE2K binds histone H3 to induce its polyubiquitination and degradation by the proteasome. Notably, ubc-20, the worm orthologue of UBE2K, also regulates both histone H3 levels and H3K9 trimethylation in C. elegans germline. Thus, our results indicate that UBE2K crosses evolutionary boundaries to promote histone H3 degradation and reduce H3K9me3 repressive marks in immortal cells.

2020-06-01 | PXD018625 | Pride

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Project description:Monkeypox virus Genome sequencing and assembly

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Polyvalent mRNA vaccination elicited potent immune response to monkeypox surface antigens

Project description:The soaring global monkeypox cases lead to a surge in demand for monkeypox vaccine, which far exceeds the supply. mRNA vaccine has achieved great success in prevention of coronavirus disease and holds promise against diverse pathogens. In this study, we generate a polyvalent lipid nanoparticle (LNP) mRNA vaccine candidate for monkeypox virus (MPXV) and evaluate its immunogenicity in animal models. This polyvalent MPXV mRNA vaccine candidate, MPXVac-097, encodes five 2022 MPXV targets that are important surface antigens. Three-dose (prime-boost-booster) MPXVac-097 vaccination elicits strong antibody response to A35R and E8L antigens, moderate response to M1R, but not B6R or A29, highlighting the differences in immunogenicity. Bulk T cell receptor (TCR) sequencing reveals preferential usage of VJ combinations and clonal expansion of peripheral T cells after MPXVac-097 vaccination. These data demonstrate initial feasibility of developing MPXV mRNA vaccine and pave the way for its future optimization.

2023-02-13 | GSE225183 | GEO

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