Transcriptomics

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Transcriptome analysis of glucose starved Bacillus subtilis cells


ABSTRACT: In this study, temporal changes in the proteome, transcriptome and extracellular metabolome of B. subtilis caused by glucose starvation were monitored. For proteomic profiling, a combination of in vivo metabolic labeling and shot gun mass spectrometric analysis was carried out for five different proteomic subfractions (i. cytosolic, ii. extracellular, iii. membrane, iv. integral membrane, and v. surface proteome fraction) leading to the identification of about 52% of the predicted proteome of B. subtilis. Acquired proteomic and transcriptomic data were analyzed using Voronoi treemaps which link functional classifications and relative expression changes of gene products according to their fate in the stationary phase. The data obtained enables for in-depth analysis of major physiological processes including protein degradation and is the first comprehensive profiling of changes in the membrane subfraction.

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

PROVIDER: GSE24058 | GEO | 2010/12/01

SECONDARY ACCESSION(S): PRJNA130141

REPOSITORIES: GEO

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