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Comparative gene expression profiling analysis of RNA-seq data for bovine macrophages infected with two Myocbacterium bovis strains of variable virulence and co-cultured with autologous lymphocytes.

ABSTRACT: In this study we compared the transcriptome of bovine macrophages infected with two Mycobacterium bovis strains of variable virulence, co-cultured with autologous lymphocytes. We used the highly virulent M. bovis strain called Mb04-303 and the attenuated Mb534 strain. We first observed that only the infection of bovine macrophages with the virulent strain, Mb04-303, induced in peripheral bovine mononuclear cells a powerful innate immune response capable of controlling the intracellular mycobacterial replication.By RNAseq analysis we found that infections with Mb04-303 downregulated the KEAP1-NFE2L2 pathway that encodes a transcriptional factor involved in antioxidant genes and inflammasome activation and upregulated the type 1 interferon signalling pathway, compared to the infections with the attenuated Mb534 strain. T

ORGANISM(S): Bos taurus

PROVIDER: GSE241059 | GEO | 2023/09/14

REPOSITORIES: GEO

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