Other

Dataset Information

0

Control of poly(A)-tail length and translation in vertebrate oocytes and early embryos


ABSTRACT: During oocyte maturation and early embryonic development, poly(A)-tail lengths strongly influence mRNA translation. However, how tail lengths are controlled at different developmental stages has been unclear. Here, we performed tail-length and translational profiling of mRNA reporter libraries (each with > 10 million 3ʹ-UTR sequence variants) in frog oocytes and embryos, and fish embryos. These analyses revealed that the UUUUA motif specifies cytoplasmic polyadenylation and identified diverse context features that modulate the activity of this 5-mer. Additional sequence motifs drive stage-specific deadenylation in embryos, and UUUUA and C-rich motifs drive tail-length-independent translational repression in oocytes. A neural network model accurately predicts tail-length change during oocyte maturation in frogs, mice, and humans. Analyses of human sequence variants showed that those predicted to disrupt tail-length control have been under negative selection, implying that our insights into control of poly(A)-tail length and translation have implications for human health and fertility.

ORGANISM(S): synthetic construct Danio rerio Xenopus laevis Mus musculus

PROVIDER: GSE241107 | GEO | 2024/02/15

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-08-08 | GSE83616 | GEO
2016-08-08 | E-GEOD-83616 | biostudies-arrayexpress
2020-01-14 | GSE134537 | GEO
2024-03-14 | GSE228001 | GEO
2014-01-29 | GSE52809 | GEO
2014-01-29 | E-GEOD-52809 | biostudies-arrayexpress
2016-08-01 | E-GEOD-83730 | biostudies-arrayexpress
2018-01-01 | GSE103719 | GEO
2022-07-22 | GSE208531 | GEO
2022-04-19 | GSE165782 | GEO