Impact of bacterial derived microvesicles on gene expression of Caco2 cells.
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ABSTRACT: Microvesicles (MEVs) were added on the Caco2 cells grown for 21 days on the insert until monolayer confluence. Triplicate samples for the MEVs treated (T1, T2, T3) and Triplicate samples for non-treated samples (C1, C2, C3) were processed for RNA extraction using Trizol method. Finally, RNA samples were DNase treated and cleaned with RNeasy MinElute Cleanup Kit (Qiagen) samples. Purified RNA was sequenced at Genome Québec (Montreal, Canada) and sequencing was performed using an Illumina NovaSeq PE100 sequencer.
ORGANISM(S): Homo sapiens
PROVIDER: GSE241164 | GEO | 2023/08/19
REPOSITORIES: GEO
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