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P300 Regulates Histone Crotonylation and Preimplantation Embryo Development

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE241196 | GEO | 2024/06/18

REPOSITORIES: GEO

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P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (RNA-Seq)

Project description:Histone lysine crotonylation is a non-acetyl histone lysine modification that is evolutionarily conserved. It plays an important role in regulating various biological processes, including gene transcriptional regulation, spermatogenesis, and cell cycle. However, the dynamic changes and functions of histone crotonylation in preimplantation embryonic development in mammals remain unclear. In this study, we have shown that the transcription coactivator P300 functions as a writer of histone crotonylation during embryonic development. Depletion of P300 resulted in significant developmental defects and dysregulation of the transcriptome of embryos. Importantly, we demonstrated that P300 catalyzes the crotonylation of histone, directly stimulating transcription and regulating gene expression, thereby ensuring successful embryo development to the blastocyst stage. Furthermore, it is showed that the histone H3 lysine 18 crotonylation (H3K18cr) modification is predominantly located in active promoter regions. It serves as an epigenetic hallmark of key transcriptional regulators and promotes the transcription activation of genes. Together, our results propose a model wherein P300-mediated histone crotonylation plays a crucial role in regulating the fate of embryonic development.

2024-06-18 | GSE241194 | GEO

P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (ChIP-Seq)

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P300 Regulates Histone Crotonylation and Preimplantation Embryo Development

Project description:P300 Regulates Histone Crotonylation and Preimplantation Embryo Development

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P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (RNA-Seq)

Project description:P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (RNA-Seq)

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P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (ChIP-Seq)

Project description:P300 Regulates Histone Crotonylation and Preimplantation Embryo Development (ChIP-Seq)

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Histone crotonylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-23 | GSE130345 | GEO

Hdac3 Interaction with p300 Histone Acetyltransferase Regulates the Oligodendrocyte and Astrocyte Lineage Fate Switch

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-02-21 | E-GEOD-76412 | biostudies-arrayexpress

Intracellular crotonyl-CoA stimulates transcription through p300-catalyzed histone crotonylation.

Project description:Acetylation of histones at DNA regulatory elements plays a critical role in transcriptional activation. Histones are also modified by other acyl moieties, including crotonyl, yet the mechanisms that govern acetylation versus crotonylation and the functional consequences of this "choice" remain unclear. We show that the coactivator p300 has both crotonyltransferase and acetyltransferase activities, and that p300-catalyzed histone crotonylation directly stimulates transcription to a greater degree than histone acetylation. Levels of histone crotonylation are regulated by the cellular concentration of crotonyl-CoA, which can be altered through genetic and environmental perturbations. In a cell-based model of transcriptional activation, increasing or decreasing the cellular concentration of crotonyl-CoA leads to enhanced or diminished gene expression, respectively, which correlates with the levels of histone crotonylation flanking the regulatory elements of activated genes. Our findings support a general principle wherein differential histone acylation (i.e., acetylation versus crotonylation) couples cellular metabolism to the regulation of gene expression.

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Maternal histone acetyltransferase KAT8 is required for porcine preimplantation embryo development.

Project description:K (lysine) acetyltransferase 8 (KAT8), an acetyltransferase that specifically catalyzes histone H4 lysine 16 acetylation, is critical for key biological processes including cell proliferation and maintenance of genome stability. However, the role of KAT8 during preimplantation development in pigs remains unclear. Results herein showed that KAT8 mRNA is maternally derived and it is required for successful development of early embryos. An abundance of KAT8 transcripts are expressed in oocytes and its abundance continuously decreases throughout meiotic maturation and preimplantation development. In addition, KAT8 expression is insensitive to RNA polymerase II inhibitor after embryonic genome activation, suggesting its maternal origin. The levels of KAT8 mRNA and H4K16 acetylation were effectively knocked down by siRNA microinjection. Knockdown of KAT8 significantly reduced the blastocyst formation rate and total cell number per blastocyst. Analysis of trophectoderm lineage and marker of DNA double-strand breaks revealed that the impaired developmental competence and quality of embryos might be attributed to defects in both the first two lineages development and genome integrity. Taken together, these results demonstrate that maternal KAT8 is indispensible for porcine early embryo development potentially through maintaining the proliferation of the first two lineages and genome integrity.

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Isoform-resolved transcriptome of the human preimplantation embryo

Project description:This SuperSeries is composed of the SubSeries listed below.

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