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Transcriptome analysis of oocytes and early embryos to demonstrate trancriptional regulation during mouse oocyte-to-embryo transition

ABSTRACT: In this study, we performed RNA-seq to elucidate how transcriptional activities of mouse oocytes and preimplantation embryos are regulated to control oocyte-to-embryo transition. Through analyzing transcriptome alterations of transgenically-modified growing oocytes, early embryos from aged female, early embryos treated with drugs disturbing proteostasis, and embryonic stem cells treated with drugs disturbing proteostasis, we identified critical roles of proteostasis in transcriptional regulation of mouse oocytes and early embryos.

ORGANISM(S): Mus musculus

PROVIDER: GSE241388 | GEO | 2025/01/20

REPOSITORIES: GEO

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Epigenetic and transcriptional regulation of mouse oocytes and early embryos

Project description:In this study, we performed ChIP-seq of RNA polymerase II, phosphorylated RNA polymerase II and related epigenetic modifications to elucidate transcriptional activities of mouse oocytes and preimplantation embryos. Through analyzing epigenetic alterations of transgenically-modified growing oocytes, early embryos from aged female, and early embryos treated with drugs disturbing proteostasis, we identified critical roles of proteostasis in transcriptional regulation of mouse oocytes and early embryos.

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Differences in oocyte composition anticipate differences in embryo quality: a proteomic study in inbred mice

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