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The identification of Stat92E target genes in Drosophila testis by ChIP-seq [CG-ChIP]

ABSTRACT: To identify the target genes of Stat92E in adult Drosophila testis cyst cells, the testes of control (c587ts) and c587ts;UAS-upd were dissected and cross-linked to conduct chromatin immunoprecipitation(ChIP) assay and ChIP-high throughput sequencing.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE241607 | GEO | 2024/09/23

REPOSITORIES: GEO

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The identification of Stat92E target genes in Drosophila testis by ChIP-seq [Stat-ChIP]

Project description:To identify the target genes of Stat92E in adult Drosophila testis cyst cells, the testes of c587ts;UAS-Stat92E were dissected and cross-linked to conduct chromatin immunoprecipitation(ChIP) assay and ChIP-high throughput sequencing.

2024-09-23 | GSE241608 | GEO

Genome-wide DNA-binding analysis for Stat92E in Drosophila testis by DamID

Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E driven by c587Gal4ts;hopTum-l

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Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis by DamID

Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E and escargot(esg) driven by c587Gal4ts

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The identification of Stat92E target genes in Drosophila testis by ChIP-seq [CG-ChIP]

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The ChIP-Sequence data of STAT92E in Drosophila adult intestines

Project description:JAK/STAT pathway plays important roles in controlling Drosophila intestinal homeostasis and regulating the ISC proliferation and differentiation. However,the downstream targets of its transcription factor-STAT92E remain largely unknown.To further identify the regualtory mechanisms of the JAK/STAT pathway in controlling intestinal homeostasis,we performed the ChIP-Seq assay with mouse raised STAT92E antibody using JAK/STAT signaling highly activated adult intestines.Through the ChIP assay, we have identified over 1000 significant peaks (p<0.01) around the putative targets.The well-characterized JAK/STAT downstream targets including Domeless,Socs36E,STAT92E and chinmo were identified in our ChIP assay,indicating that our experiment is workable to identify novel JAK/STAT downstream targets in adult intestines.This work will provide insights into our understanding of regulatory mechanisms of JAK/STAT signaling during Drosophila intestinal development. Identify the ChIP peaks of STAT92E antibody using JAK/STAT signaling highly actived Drosophila adult intestines, compared with input libaray as the control

2015-05-12 | E-GEOD-67346 | biostudies-arrayexpress

Stat92E-GFP_12-24h_embryonic_ChIP-seq

Project description:modENCODE_submission_5014 This submission comes from a modENCODE project of Kevin White. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The White Lab is aiming to map the association of all the Transcription Factors (TF) on the genome of Drosophila melanogaster. One technique that we use for this purpose is chromatin immunoprecipitation coupled with deep sequencing (ChIP-seq) utilizing an Illumina next generation sequencing platform. The data generated by ChIP-seq experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested TF. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Stat92E-GFP; Developmental Stage: Embryo 12-24h; Genotype: PBac{y[+]-attP-3B}VK00037; Transgene: Stat92E genomic coding region; EXPERIMENTAL FACTORS: Developmental Stage Embryo 12-24h; Target gene Stat92E; Strain Stat92E-GFP; Antibody GFP ab290 (target is Green Fluorescent Protein)

2013-08-11 | E-GEOD-49775 | biostudies-arrayexpress

The ChIP-Sequence data of STAT92E in Drosophila adult intestines

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Project description:The identification of Stat92E target genes in Drosophila testis by ChIP-seq [Stat-ChIP]

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The identification of Stat92E target genes in Drosophila testis by ChIP-seq

Project description:The identification of Stat92E target genes in Drosophila testis by ChIP-seq

| PRJNA1008790 | ENA

modENCODE_White Lab: genome-wide ChIP data of Stat92E from WPP on Illumina Genome Analyzer.

Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains genome-wide binding profile of the factor Stat92E from WPP generated by ChIP and analyzed on Illumina Genome Analyzer. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

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