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Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k4me3_wt_col_seedlings_db)

ABSTRACT: a2e_fie - h3k4me3_wt_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE24162 | GEO | 2011/03/28

SECONDARY ACCESSION(S): PRJNA133679

REPOSITORIES: GEO

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Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k4me3_fie_col_seedlings_db)

Project description:a2e_fie - h3k4me3_fie_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip

2011-03-28 | GSE24161 | GEO

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k27me3_wt_col_seedlings_db)

Project description:a2e_fie - h3k27me3_wt_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip

2011-03-28 | GSE24160 | GEO

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k27me3_fie_col_seedlings_db)

Project description:a2e_fie - h3k27me3_fie_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip

2011-03-28 | GSE24159 | GEO

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k27me3_fie_col_seedlings_db)

Project description:a2e_fie - h3k27me3_fie_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip 4 dye-swap - CATMA arrays

2011-03-28 | E-GEOD-24159 | biostudies-arrayexpress

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k27me3_wt_col_seedlings_db)

Project description:a2e_fie - h3k27me3_wt_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip 4 dye-swap - CATMA arrays

2011-03-28 | E-GEOD-24160 | biostudies-arrayexpress

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k4me3_fie_col_seedlings_db)

Project description:a2e_fie - h3k4me3_fie_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip 4 dye-swap - CATMA arrays

2011-03-28 | E-GEOD-24161 | biostudies-arrayexpress

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant (h3k4me3_wt_col_seedlings_db)

Project description:a2e_fie - h3k4me3_wt_col_seedlings_db - Epigenomic mapping in fie - Genome-wide epigenomic mapping Keywords: chip-chip 4 dye-swap - CATMA arrays

2011-03-28 | E-GEOD-24162 | biostudies-arrayexpress

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-03-28 | GSE24163 | GEO

Genome-wide analysis of H3K27me3 and H3K4me3 deposition in the fie mutant

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-03-28 | E-GEOD-24163 | biostudies-arrayexpress

Genome wide analysis of the chromatin state and gene expression in Wild-type, tor-es mutant, and fie mutants

Project description:Using Western blot, we found the level of H3K27me3, but not H3K4me3, H3K9me2 and H3K36me3, was specifically reduced in the tor-es mutant. To gain a genome-wide view of the effects of TOR activity on H3K27me3 distribution, we performed quantitative chromatin immunoprecipitation with an exogenous reference genome (ChIP-Rx) followed by deep-sequencing. We find a global reduction of H3K27me3 occupancy in tor-es, whereas the H3K9me2 level was largely unaffected. These results suggest that TOR may be a specific and direct regulator of global deposition of H3K27me3. To investigate the function of TOR phosphorylation of FIE, we complemented the heterozygous fie/+ plants with GFP-FIE or the phosphorylation site mutant (SSTS/AAAA) under the control of the FIE promoter. To provide a parallel comparison with SSTS/AAAA/fie, we generated estradiol-inducible fie-amiR-es transgenic lines, that eliminated FIE protein. Quantitative ChIP-seq analyses revealed greatly reduced H3K27me3 levels across the genome in SSTS/AAAA and fie-amiR-es mutants. And transcriptome profiling by RNA-Seq was conducted to globally identify thousands of genes coordinately dysregulated in the shoots of SSTS/AAAA and fie-amiR-es plants. Furthermore, gene Ontology analysis of 986 TOR-FIE-PRC2 target genes revealed significant enrichment for transcription factors/regulators controlling a broad spectrum of developmental programs.

2022-06-20 | GSE161807 | GEO

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