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Dynamic analysis of mononuclear transcription profiles of fat in different parts of mice during aging

ABSTRACT: Adipose tissue shows significant changes during aging. Here, we used single-nucleus RNA-seq to map the single-nucleus transcription profiles of mouse subcutaneous adipose tissue ( SAT ) and visceral adipose tissue ( VAT ) at a single-nucleus resolution, showing the differences in visceral and subcutaneous adipose tissue changes with aging. The mononuclear sequencing method enabled us to restore all major cell types in mouse white adipose tissue, and we characterized adipocytes, immune cells, and preadipocytes. We demonstrated the existence of different adipocyte subsets and showed that aging leads to a decrease in adipogenic subsets. In addition, with aging, both subcutaneous adipose tissue and visceral adipose tissue showed a decrease in immune mechanisms.

ORGANISM(S): Mus musculus

PROVIDER: GSE241718 | GEO | 2024/07/17

REPOSITORIES: GEO

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Dynamic analysis of mononuclear transcription profiles of fat in different parts of mice during aging

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