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Live tracking of basal stem cells of the epidermis during growth, homeostasis, and injury response in zebrafish

ABSTRACT: Basal stem cells of the epidermis continuously differentiate into keratinocytes and replenish themselves via self-renewal to maintain skin homeostasis. Numerous studies have attempted to reveal how basal cells undergo differentiation or self-renewal; however, this has been hampered by a lack of robust basal cell markers and analytical platforms that allow single-cell tracking. Here, we report integrin b4 (itgb4) is a useful marker for basal cell labelling, irrespective of the body region, stage, and regenerative status. We employed Cre-loxP recombination in combination with live cell tracking of single basal clones in the caudal fin and investigated the embryonic origin and behaviour of basal cells during fish growth and homeostasis. Although most basal cells, including those in fins, became quiescent in the adult stage, genetic cell ablation showed that basal cells were reactivated to either self-renew or differentiate, depending on the injured cell type. Our findings provide a platform for quantitative in vivo imaging of basal stem cells applicable at wider stages and under various conditions and reveal unanticipated basal cell dynamism in response to distinct wound signals.

ORGANISM(S): Danio rerio

PROVIDER: GSE241757 | GEO | 2023/09/06

REPOSITORIES: GEO

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Distinct Self-renewal and Differentiation Phases in the Niche of Infrequently Dividing Hair Follicle Stem Cells

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Expression profiling of prospero mutant VNC cells in Drosophila embryos

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ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium

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