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KaedeGreen and Red CD8 T cells from vaccinia-GP33 infected skin

ABSTRACT: In order to determine transcriptional differences between cells in the draining lymph node and those recently emigrating from the skin, we performed single cell RNA sequencing on KaedeGreen and KaedeRed CD8 T cells 12 days post infection with vaccinia-GP33. CD8+ cells were MACS enriched. Cells were stained with two tetramers (H2-Kb-B8R20-27 and H2-Db-GP33-41) conjugated to APC. Cells were then incubated with TotalSeq anti-APC (Biolegend, 408009)

ORGANISM(S): Mus musculus

PROVIDER: GSE241820 | GEO | 2024/01/30

REPOSITORIES: GEO

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