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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [ChIP-seq]

ABSTRACT: Sumoylation alters the transcriptional activity of many of the transcription factors known to regulate CSCs/TICs and epithelial-mesenchymal transition (EMT). To identify transcription factor involved in the growth effects mediated by SUMO inhibition, Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) was used to evaluate genomic occupancy by SUMO1 and Etv1.

ORGANISM(S): Mus musculus

PROVIDER: GSE242484 | GEO | 2025/03/16

REPOSITORIES: GEO

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [CUT&RUN]

Project description:Sumoylation alters the transcriptional activity of many of the transcription factors known to regulate CSCs/TICs and epithelial-mesenchymal transition (EMT). To identify transcription factor involved in the growth effects mediated by SUMO inhibition, Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) was used to evaluate genomic occupancy by HA-tagged Etv1.

2025-03-16 | GSE272057 | GEO

SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [RNA-seq]

Project description:RNA-seq was used to compare the effect of SUMOylation inhibition, and Etv1 knockdown on Ube2iF/F cell line transcriptiome. 5xKR or 5xKR-S1 was expressed to examine differential phenotypic transcriptiome changes.

2025-03-16 | GSE242485 | GEO

SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis

Project description:SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [scRNA-seq]

Project description:SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [scRNA-seq]

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [RNA-seq]

Project description:SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [RNA-seq]

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [CUT&RUN]

Project description:SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [CUT&RUN]

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [ChIP-seq]

Project description:SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [ChIP-seq]

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SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [scRNA-seq]

Project description:scRNA-seq was used to compare the cell populations and transcriptiome in 6-week-old and 10-week-old FVB, MMTV-NIC, MMTV-NIC-Ube2ifl/fl mice

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A SUMOylated C-terminus fragment of glutamate transporter EAAT2 linked to inherited ALS mediates motor axon impairment

Project description:Downregulation of expression and activity levels of the astroglial glutamate transporter EAAT2 is thought to be implicated in motor neuron excitotoxicity in amyotrophic lateral sclerosis (ALS). We previously reported that EAAT2 is cleaved by caspase-3 at the cytosolic C-terminus domain, impairing the transport activity and generating a proteolytic fragment found to be SUMO1 conjugated (CTE-SUMO1). We show here that this fragment accumulates in the nucleus of spinal cord astrocytes in vivo throughout the disease stages of the SOD1-G93A mouse model of ALS. In vitro expression in spinal cord astrocytes of the C-terminus peptide of EAAT2 (CTE), which was artificially fused to SUMO1 (CTE-SUMO1fus) to mimic the endogenous SUMOylation reaction, recapitulates the nuclear accumulation of the fragment seen in vivo and causes caspase-3 activation and axonal growth impairment in motor neuron-derived NSC-34 cells and primary motor neurons co-cultured with CTE-SUMO1fus-expressing spinal cord astrocytes. This indicates that CTE-SUMO1fus could trigger non-cell autonomous mechanisms of neurodegeneration. Prolonged nuclear accumulation of CTE-SUMO1fus in astrocytes leads to their degeneration, although the time frame of the cell-autonomous toxicity is longer than the one for the indirect toxic effect on motor neurons. As more evidence on the implication of SUMO substrates in neurodegenerative diseases emerges, our observations strongly suggest that the nuclear accumulation in spinal cord astrocytes of a SUMOylated proteolytic fragment of the astroglial glutamate transporter EAAT2 could take part to the pathogenesis of ALS and suggest a novel, unconventional role for EAAT2 in motor neuron degeneration in ALS. Comparison is made between the toxic fragment (SUMO) and the same fragment without lysines that can be sumo-ylated (CTE). Four replicates have been performed for each sample group.

2011-07-19 | E-GEOD-26070 | biostudies-arrayexpress

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