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Characterize compounds that affect V(D)J recombination and chromsomal translocation using MP-HTGTS

ABSTRACT: Chromosome translocation plays a crucial role in the development of tumors, but its underlying biological mechanisms, especially in human cells, remain poorly understood. In this study, we aimed to shed light on the mechanisms governing chromosome translocation in K562 cells. To investigate this, we initiated the process by inducing two double-strand breaks (DSBs) using Cas9 sgRNA and subsequently quantified the frequency of chromosome translocation events. Furthermore, we explored the influence of several compounds by introducing them alongside the DSBs, allowing us to assess their impact on chromosome translocation. Our research unveiled the identification of two noteworthy compounds, HDAC inhibitors and HSP90 inhibitors, which were found to significantly enhance the occurrence of chromosome translocation. These findings contribute to a deeper understanding of the molecular processes involved in tumorigenesis and provide potential targets for future therapeutic interventions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE242740 | GEO | 2023/12/30

REPOSITORIES: GEO

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Characterize compounds that affect V(D)J recombination and chromsomal translocation using MP-HTGTS

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