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EHUA Vibrio alginolyticus EPGS Nimblegen

ABSTRACT: Vibrio alginolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. In this study, Vibrio alginolyticus wild type strain EPGS is compared with the mutants of Ser-Thr kinase PpkA and phosphatase PppA, after cultured for 7h, in Luria-Bertani containing medium 3 % NaCl at 30 C. Our goal is to determine the ppkA and pppA regulon.

ORGANISM(S): Vibrio parahaemolyticus RIMD 2210633 Vibrio alginolyticus

PROVIDER: GSE24319 | GEO | 2011/12/31

SECONDARY ACCESSION(S): PRJNA132843

REPOSITORIES: GEO

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EHUA Vibrio alginolyticus EPGS Nimblegen

Project description:Vibrio alginolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. In this study, Vibrio alginolyticus wild type strain EPGS is compared with the mutants of Ser-Thr kinase PpkA and phosphatase PppA, after cultured for 7h, in Luria-Bertani containing medium 3 % NaCl at 30 C. Our goal is to determine the ppkA and pppA regulon. Three wild type and five mutant Vibrio alginolyticus samples were compared.

2011-12-31 | E-GEOD-24319 | biostudies-arrayexpress

RIMD 2210633 wild type versus vtrA or vtrB mutants

Project description:Vibrio parahaemolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Vibrio parahaemolyticus wild type strain RIMD 2210633 compared with the mutants of VtrA and VtrB have a winged helix-turn-helix DNA binding motif that genes encoded on pathogenicity island loci, at OD600=1.0 in Luria-Bertani containing medium 0.5 % NaCl at 37˚C. Our goal is to determine the VtrA or VtrB regulon.

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Vibrio alginolyticus iTRAQ

Project description:In recent years, due to the influence of climate change and rising sea temperature, the incidence of Vibrio alginolyticus infections is increasing, and becoming the second most common Vibrio species reported in human illness. Therefore, better understanding of the pathogenic mechanism of V. alginolyticus infection is urgently needed. Vvrr1 (Vibrio virulence regulatory RNA 1) is a new found ncRNA predicted to be closely related to the adhesion ability of V. alginolyticus through the previous RNA-seq. In this study, the target genes of Vvrr1 were fully screened and verified by constructing Vvrr1 over-expressed strains and proteome sequencing technology.

2020-05-26 | PXD014375 | Pride

RIMD 2210633 wild type versus vtrA or vtrB mutants

2010-07-16 | E-GEOD-17242 | biostudies-arrayexpress

Vibrio parahaemolyticus gene expression affected by 0.04% bile

Project description:Vibrio parahaemolyticus is a Gram-negative marine bacterium. Strain RIMD 2210633, the wild type strain of the organism, causes acute gastroenteritis in humans. Human intestinal factor bile often affects the global gene regulation in some species of enteropathogenic bacteria. To determine the genes in V. parahaemolyticus that respond to bile, we investigated the differences in the transcriptomes of the wild type strain and the vtrA-null strain grown in Luria-Bertani medium cultivated with or without 0.04% crude bile. The vtrA gene encodes the previously identified T3SS2 regulator. Our goal is to demonstrate bile regulon controlled by VtrA in V. parahaemolyticus.

2010-10-10 | GSE21666 | GEO

Virulence of Vibrio alginolyticus as determinants of immune outcomes in the oyster Crassostrea hongkongensis

Project description:Vibrio species distribute ubiquitously in marine and coastal environments, with implications for severe infectious diseases in human and marine animals. However, precisely what defensive strategies the host employ against Vibrio pathogens with distinct virulence remain poorly understood. Being an ecologically relevant host, the oyster Crassostrea hongkongensis serves as an excellent model for elucidating mechanisms underlying host-Vibrio interactions. In this study, we generated one mutant Vibrio alginolyticus strains (V. alginolyticus△VSCC) with attenuated virulence by knocking out the VSCC encoding gene, one of the core components of type III secretion systems (T3SSs), based on the evidence that V. alginolyticus△VSCC infection leads to a marked reduction in the apoptotic rate of hemocyte hosts, compared to V. alginolyticusWT control. In comparative proteomics, it was revealed that distinct immune responses were elicited upon encounter with V. alginolyticus strains of different virulence. Quite strikingly, the peroxisomal and apoptotic pathways are activated by V. alginolyticusWT infection, whereas phagocytosis and cell adhesion were enhanced in V. alginolyticus△VSCC infection. Collectively, we conclude that adaptation in host immune responses is determined in part by pathogen virulence, which safeguards elimination of invading bacteria in efficient and timely manners.

2022-02-16 | PXD022551 | Pride

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Project description:Comparative proteomics analysis of type VI secretion-dependent secretomes of Vibrio alginolyticus 12G01

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Project description:RNA-seq analysis demonstrated that VqsA (18672) controls ~275 genes' expression in V. alginolyticus. Collectively, our data established that VqsA plays essential roles in QS regulation and may facilitate the illumination of the mechanisms bacterial cells sense environmental signals and integrate them into coordinated QS responses.

2021-12-06 | GSE125760 | GEO

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