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Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

ABSTRACT: The preoptic area of hypothalamus plays an important role in sleep homeostasis. We used snRNA-seq to identify neurons involved in homeostatic sleep response.

ORGANISM(S): Mus musculus

PROVIDER: GSE243489 | GEO | 2024/04/01

REPOSITORIES: GEO

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Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

Project description:Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

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Sleep Deprivation and Recovery Sleep for 7 Regions of Mouse Brain.

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Project description:Sleep loss regulates gene expression throughout the brain and impacts learning and memory. However, the molecular consequences of sleep deprivation and the ability of subsequent sleep (recovery sleep) to restore baseline gene expression remain underexplored. Our goal here is to overview transcriptional changes at the gene level in the cortex of adult male wildtype mice in response to sleep loss and recovery sleep. This dataset constitutes an integration of novel data with two publicly available RNA-seq studies and contains 3, 5, and 6 hours of sleep deprivation and 2 and 6 hours of recovery sleep time points.

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RNA-Seq analysis of galaninergic neurons from ventrolateral preoptic nuleus identifies expression changes between sleep and wake

Project description:Galanin neurons at ventrolateral preoptic nuleus (VLPO) have been hypothesized to be sleep-active neurons. With the use of laser capture microdissection (LCM), we specifically isolated single galanin neurons, and our goal was to assess expression changes in VLPO galanin cells between sleeping mice and mice kept awake using gentle handling.

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Project description:GABAergic transmission controls cortical sleep, however the molecular determinants of GABAergic control of this phenomenon are unknown. We used immunoprecipitation of the inhibitory postsynaptic protein gephyrin to identify gephyrin interactors at ZT6 (sleep) and ZT6 with sleep deprivation (ZT6SD).

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Altered hippocampal transcriptome dynamics following sleep deprivation

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