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Time-course leaf expression data from Populus deltoides

ABSTRACT: We conducted micro-array analysis to quantify the global transcriptome variations in leaves through the course of the year allowing for identification of changing developmental signals. We used RNA samples from pre-formed and mature leaves in the upper crown of a sexually mature Populus deltoides tree 2 hours after sunrise.

ORGANISM(S): Populus sp. Populus deltoides

PROVIDER: GSE24349 | GEO | 2011/05/23

SECONDARY ACCESSION(S): PRJNA132797

REPOSITORIES: GEO

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Time-course leaf expression data from Populus deltoides

Project description:We conducted micro-array analysis to quantify the global transcriptome variations in leaves through the course of the year allowing for identification of changing developmental signals. We used RNA samples from pre-formed and mature leaves in the upper crown of a sexually mature Populus deltoides tree 2 hours after sunrise. Three independent samples of leaf/pre-formed leaf tissues were collected from the upper crown of a single sexually mature tree. RNA was extracted from tissues and hybridized on Affymetrix Genechip Poplar Genome Array. Tissues represented two batches (batch 1 from 2005 and batch 2 from 2006). Therefore, March samples were used to normalize the other month samples within the same batch.

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Gene expression in leaves of a Populus trichocarpa and Populus deltoides pseudo-backcross population

Project description:A microarray analysis of whole-genome gene expression in leaves was carried out in a (Populus trichocarpa X Populus deltoides) X Populus deltoides pseudo-backcross pedigree. Genetic variation in gene expression was quantified for 55,793 predicted gene models based on a single probe per gene. Resultant data contributed to the analysis of the genetic architecture of gene expression in leaves of Populus. Data include one biological replicate of 183 individuals segregating from a pseudo-backcross pedigree of (Populus trichocarpa X Populus deltoides) X Populus deltoides analyzed for gene expression (GE) in roots using one probe per gene for 55793 independent gene models (probes E_POPLARSxxxxxPxxxxx) and single feature sequence polymorphism (SFP) using one probe per gene for 12084 independent gene models (probes G_POPLARSxxxxxPxxxxx). GE and SFP probes were selected from 6-7 probes per gene previously tested in a pilot study of the two parent trees of the cross (Populus deltoides X Populus trichocarpa)

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Project description:Populus deltoides and Populus trichocarpa were exposed to either ambient air or an acute ozone exposure of 200 ppb for 9 hrs and ozone response was profiled for each genotype by hybridising control against ozone-exposed samples per genotype. Keywords: stress response, genotype comparrison, ozone exposure

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Gene expression in whole-roots of a Populus trichocarpa and Populus deltoides pseudo-backcross population

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Gene expression and intragenic polymorphism in a Populus trichocarpa and Populus deltoides pseudo-backcross pedigree

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Gene expression in whole-roots of a Populus trichocarpa and Populus deltoides pseudo-backcross population

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Identification of rust-induced genes in poplar using Populus 2.0 NimbleGen oligoarray

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