Effects of csr-1 knockdown on gene expression and mRNA splicing
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ABSTRACT: The Integrator is an evolutionarily conserved protein complex that functions to process the 3’ maturation of small nuclear RNA molecules that are an essential part of the spliceosome. We performed a genome-wide RNAi screen in the C. elegans system and identified csr-1 as an essential gene required for this process. Knockdown of csr-1 activates an snRNA misprocessing reporter and causes aberrant 3’ cleavage of several endogenous snRNA transcripts. Using whole-transcriptome RNA sequencing, we evaluated the effects of csr-1 knockdown via RNAi relative to empty vector control and found that knockdown of csr-1 causes aberrant expression of various snRNA transcripts along with significant changes to alterantive splicing of ~400 transcripts. Overall, this experiment identified the role of csr-1 as a novel regulator of snRNA processing and profiled its impact on gene expression and alternatively splicing at the transcriptome level.
ORGANISM(S): Caenorhabditis elegans
PROVIDER: GSE243495 | GEO | 2024/05/06
REPOSITORIES: GEO
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