Transcriptomics

Dataset Information

0

MTORC2-mediated cell-cell interaction promote BMP4-induced WNT activation and mesoderm differentiation


ABSTRACT: The mechanistic target of rapamycin complex 2 (mTORC2) is essential for embryonic development but the underlying molecular mechanisms remain unclear. Here we show that disruption of mTORC2 in human embryonic stem cells (hESCs) considerably alters the balance of Rho/Rac signaling and reduces cell adhesion. Although these changes have no clear effect on their self-renewal and the expression of pluripotent markers, they significantly impede BMP-induced activation of canonical WNT genes, leading to impaired mesendoderm differentiation. Direct activation of the downstream WNT pathway by inhibiting GSK3 dramatically improves mesendoderm differentiation in mTORC2-deficient hESCs. Our studies uncover a new mechanism by which mTORC2 regulates cell fate determination and link the intercellular contacts with the activation of WNT genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE243723 | GEO | 2023/11/06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-11-29 | GSE72545 | GEO
2012-10-17 | E-GEOD-30125 | biostudies-arrayexpress
2012-10-17 | GSE30125 | GEO
2024-03-20 | GSE235207 | GEO
2019-03-29 | PXD013061 | Pride
2023-02-23 | E-MTAB-12598 | biostudies-arrayexpress
2022-09-30 | E-MTAB-12075 | biostudies-arrayexpress
2020-01-15 | PXD015824 | Pride
2022-09-30 | E-MTAB-12076 | biostudies-arrayexpress
2024-09-02 | BIOMD0000000581 | BioModels