Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stress regulates Alzheimer's Disease progression via selective enrichment of CD8+ T cells

ABSTRACT: This study investigates stress's impact on Alzheimer's disease (AD) using male APP/PS1 transgenic mice. Negative stressors (chronic social defeat, restraint) and positive hedonia (environmental enrichment, EE) were applied. Stress worsens AD pathology, while EE slows progression. Brain RNA-seq reveals IL-6 and IL-10 as key stress-related AD regulators. Flow cytometry shows CD8+/CD4+ T cell ratio shifts in response to SE and EE. SE increases CD8+/CD4+ ratio, opposite in EE. Depletion and enrichment of CD8+ T cells both accelerate AD, indicating immune intervention's negative impact. Stress management and balanced immunity may aid AD therapy, highlighting novel potential treatment.

ORGANISM(S): Mus musculus

PROVIDER: GSE244131 | GEO | 2023/12/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparison of CD4+ and CD8+ T cells from spleen of mice raised in standard or in enriched environment.

Project description:C57BL6/J female mice were raised either in standard environment (SE) for 8 weeks after birth or were put in enriched environment (EE) at postnatal 4 weeks up to 8 weeks old. Mice were sacrificed at 8 weeks and spleen were harvested. CD4+ and CD8+ T cells were sorted by FACS and CD4+ or CD8+ T were profiled using a low-input protocol adapted from single-cell RNAseq.

2018-05-28 | GSE100584 | GEO

Comparison of CD4+ and CD8+ T cells from choroid plexus of mice raised in standard or in enriched environment.

Project description:C57BL6/J female mice were raised either in standard environment (SE) for 8 weeks after birth or were put in enriched environment (EE) at postnatal 4 weeks up to 8 weeks old. Mice were sacrificed at 8 weeks and choroid plexus in the brain were harvested. CD4+ and CD8+ T cells were sorted by FACS and 5-20 CD4+ or CD8+ T were profiled using a low-input protocol adapted from single-cell RNAseq.

2018-05-28 | GSE100586 | GEO

Analysis of clinical characteristics of colorectal cancer patients and rehabilitation study of environment-rich training

Project description:Interventions: Healthy control group:none;CRC group:Conventional drug therapy;Autonomous Sports Group:Home-based voluntary exercise training as part of regular treatment;EE group:Home based voluntary exercise training combined with environmental enrichment EE training on top of conventional treatment Primary outcome(s): Physical Effort Scale (PA); depression (SDS) scale;anxiety (SAS) scale;perceived stress scale;Pittsburgh Sleep Quality Index Scale (PSQI); Piper Fatigue Modified Scale (RPFS);Piper Fatigue Modified Scale (RPFS);Colorectal Cancer Quality of Life Specific Scale (EORTC QLQ-CR38) Study Design: Parallel

| 87692 | ecrin-mdr-crc

Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 mice [MeDIP-seq]

Project description:Chronic stress episodes increase metabolic disease risk even after recovery. We propose that persistent stress detrimentally impacts hepatic metabolic reprogramming, particularly mitochondrial function. In male C57BL/6 mice chronic variable stress (Cvs) reduced energy expenditure (EE) and body mass despite increased energy intake versus controls. This coincided with decreased glucose metabolism and increased lipid β-oxidation, correlating with EE. After Cvs, mitochondrial function revealed increased thermodynamic efficiency (ƞ-opt) of complex CI, positively correlating with blood glucose and NEFA and inversely with EE. After Cvs recovery, the metabolic flexibility of hepatocytes was lost. Reduced CI-driving NAD+/NADH ratio, and diminished methylation-related one-carbon cycle components hinted at epigenetic regulation. Although initial DNA methylation differences were minimal after Cvs, they diverged during the recovery phase. Here, the altered enrichment of mitochondrial DNA methylation and linked transcriptional networks were observed. In conclusion, Cvs rapidly initiates the reprogramming of hepatic energy metabolism, supported by lasting epigenetic modifications.

2024-03-11 | GSE235423 | GEO

Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 mice [MTA array, Affymetrix]

2024-03-11 | GSE234944 | GEO

Dysfunctional epidermis increases dermis inflammation in systemic sclerosis: results from a novel epidermal equivalent engineered from SSc keratinocytes.

Project description:In systemic sclerosis (SSc) evidence suggests abnormal keratinocyte-fibroblast interactions. We investigated the potential epidermal dysfunction in SSc and its effects on dermis homeostasis. Epidermal equivalents (EE) were generated from six healthy donor (HD) and four SSc keratinocytes. Skin and EE expression of proliferation, differentiation, and activation markers was evaluated by immunohistochemistry. The transcriptomic profile of SSc-EE and HD-EE was identified by RNAseq analysis. EE conditioned medium (CM) was used to stimulate fibroblasts, and their production of interleukin (IL)-6, IL-8, matrix metalloproteinase (MMP)-1, type-I collagen (col-I), and fibronectin was assessed by ELISA. Compared to HD, SSc-EE exhibited aberrant differentiation, enhanced expression of activation markers, and lower mitotic rate of basal keratinocytes, reproducing most of the abnormalities observed in SSc epidermis. RNAseq analysis revealed that, compared to HD-EE, SSc-EE were characterized by the downregulation of HOX gene family members and by the upregulation of metabolic and oxidative stress molecular pathways. EE-CM enhanced the fibroblast production of IL-6, IL-8, MMP-1, Col-I, and fibronectin (p<0.05). Except for Col-I and fibronectin, this effect was 2-fold higher in the presence of CM generated form SSc-EE. IL-1 was, at least in part, responsible for keratinocyte-dependent fibroblast activation. SSc-EE recapitulate the in vivo characteristic of SSc epidermis demonstrating that SSc keratinocytes have an intrinsically altered differentiation program possibly due to the downregulation of genes from the HOX family. The increased metabolic and oxidative stress associated with SSc epidermis may participate to dermis chronic inflammation and fibrosis

2021-08-13 | GSE156173 | GEO

Multi-center clincal study of umbilical cord blood cytokine-induced killer cells (U-CIKs) infusion on influence of the immune function in Advanced Cancer after Chemotherapy

Project description:Interventions: A:umbilical cord blood cytokine-induced killer cells;B:Routine radiotherapy Primary outcome(s): NK cell population;CD4/CD8 ratio;Contents of plasma immunoglobulin Study Design: Non randomized control

| 2669506 | ecrin-mdr-crc

Effect of Perioperative Immune Status and Long-term Prognosis of Thymosin Alpha-1 for Injection(Zadaxin) in Treatment of Patients with Colorectal Cancer: A Prospective, Randomized Controlled Trial

Project description:Interventions: Test group:Thymosin Alpha-1 subcutaneous injection;Control group:without Thymosin alpha-1 Primary outcome(s): CD4 / CD8 ratio;CRP;PLR;NLR;1-year DFS;3-year DFS;2-year OS Study Design: Parallel

| 36170 | ecrin-mdr-crc

A controlled trail of Autologous and umbilical cord blood cytokine-induced killer cells (U-CIKs) infusion on influence of the immune function in Advanced Cancer after Radiotherapy

Project description:Interventions: A:Autologous blood cytokine-induced killer cells;B:umbilical cord blood cytokine-induced killer cells;C:Routine treatment Primary outcome(s): NK cell population;CD4/CD8 ratio;Contents of plasma immunoglobulin Study Design: Non randomized control

| 2669507 | ecrin-mdr-crc

Immediate dysfunction of vaccine-elicited CD8+ T cells primed in the absence of CD4+ T cells

Project description:CD4+ T cell help is critical for optimal CD8+ T cell expansion after priming in many experimental systems. However, a role for CD4+ T cells in regulating the initial steps of CD8+ T cell effector differentiation is not well established. Here we demonstrate that absence of CD4+ T cells at the time of replication-incompetent adenovirus vector immunization of C57BL/6 mice led to immediate CD8+ T cell dysfunction characteristic of exhaustion at the first detectable timepoints as well as impaired expansion of antigen-specific CD8+ T cells. The absence of CD4+ T cell help resulted in antigen-specific CD8+ T cells that had reduced ex vivo cytotoxicity and decreased capacity to produce IFN-Î³ and TNF-Î±. CD8+ T cells primed in the absence of CD4+ T cells expressed elevated levels of the inhibitory receptors PD-1, LAG-3, and Tim-3, and these cells exhibited transcriptomic exhaustion profiles by gene set enrichment analysis. This dysfunctional state was imprinted within 3 days of immunization and could not be reversed by provision of CD4+ T cell help after priming. Partial rescue of unhelped CD8+ T cell expansion and effector differentiation could be achieved by PD-1 pathway blockade or recombinant IL-2 administration. This study identifies a novel, previously undescribed role of CD4+ T cells to prevent immediate dysfunction and features of exhaustion in CD8+ T cells following antigen priming. Splenic AL11-specific CD8 T cells from mice immunized with Ad5HVR48(1-7)-SIV Gag and treated with anti-CD4 antibody or not were purified by FACS on day 14 post-immunization

2016-07-01 | E-GEOD-73001 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data